FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948473
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40340.4253152171964832No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37330.39357999647855024No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCCA36440.3841964926782312No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32110.3385441652002745No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTCGAGCCCAG32090.33833329994633476No Hit
CTCCTGCACTGGGACCAGCTCCAACATCGGGGCAGGTTATGAAGTACACTGGTACCAGCAGGTTCCAGGAACAGC27460.28951799365928177No Hit
GGCCCAGGCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC22860.24101898525313845No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22770.24007009161040957No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22270.23479846026191573No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC21880.23068658781009052No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGCGGCCTGGGCCCAGG21720.2289996657785725No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20550.21666404842309692No Hit
GTCCTATGACAGCGGCGTGATTGGGGTTTGGCTCTTCGGCGGAGGGACCA19820.20896746665429589No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19120.20158718276640453No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19080.201165452258525No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18670.1968427145527601No Hit
GGCCATCACTGGGCTCGAGGCTGAGGATGAGGCTGATTATTACTGCCAGT18150.19136021795032648No Hit
GGTTATGAAGTACACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGG17980.18956786329183856No Hit
CTGCACAGCGGCCTGGGCCCAGGCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGATCAC17930.18904070015698918No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAGAG17580.1853505582130435No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGATCAC17350.1829256077927363No Hit
GATTATTACTGCCAGTCCTATGACAGCGGCGTGATTGGGGTTTGGCTCTTCGGCGGAGGGACCAAGCTGACCGT17070.17997349423757977No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17070.17997349423757977No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17060.17986806161060992No Hit
ATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG16650.17554532390484492No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16130.17006282730241135No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16130.17006282730241135No Hit
GGGCAGAGGATCACCATCTCCTGCACTGGGACCAGCTCCAACATCGGGGCAGGTTATGAAGTACACTGGTACCAG16060.1693247989136222No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16050.16921936628665235No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15980.1684813378978632No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCCAAACCCCAATC15880.16742701162816442No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15750.16605638747755602No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15710.16563465696967652No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15660.16510749383482715No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15530.16373686968421874No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15250.1607847561290622No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14730.1553022595266286No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13670.14412640106782165No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12940.13642981929902065No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAACCTGCTGGTA12840.13537549302932186No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTCCAGGGAGA12740.1343211667596231No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12710.13400486887871346No Hit
GTGTCTGGGGCCCCAGGGCAGAGGATCACCATCTCCTGCACTGGGACCAG12680.13368857099780382No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12670.13358313837083396No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCGGCGTGATTGGGG12510.13189621633931595No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC12400.13073645744264728No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGCGG12370.13042015956173766No Hit
GAAGTACACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCAT12020.12673001761779198No Hit
GTACACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCATCTA11670.12303987567384629No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT11630.12261814516596678No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11520.12145838626929813No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11330.11945516635687047No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11240.11850627271414156No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11230.1184008400871717No Hit
GTTATGAAGTACACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGGC11200.11808454220626205No Hit
GCTTGGTCCCTCCGCCGAAGAGCCAAACCCCAATCACGCCGCTGTCATAGGACTGGCAGTAATAATCAGCCTCAT11160.11766281169838255No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11120.11724108119050304No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGCGGCCTGGGC10830.11418353500837661No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10560.11133685408018995No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10540.1111259888262502No Hit
CTTCATAACCTGCCCCGATGTTGGAGCTGGTCCCAGTGCAGGAGATGGTGATCCTCTGCCCTGGGGCCCCAGACA10450.1101770951835213No Hit
GTCTCTGGTGGCTCCGTCACCAGTGATGGTTTCTCCTGGAGTTGGATCCG10420.10986079730261167No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCGAGGCTGAGGATG10410.1097553646756418No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTC10320.1088064710329129No Hit
GTGCAGGAGATGGTGATCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG10210.10764671213624427No Hit
CTCACTGCACAGCGGCCTGGGCCCAGGCTGTGCTGACGCAGCCGCCCTCA10030.10574892485078646No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9830.10364027231138893No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGCGGCCTGGGCCCAGGCTGTG9800.10332397443047932No Hit
TGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC9660.10184791765290102No Hit
GTATTACTGTGCCAGAATGTATTTCTTTGATACTAGTGGTTCCGGCCCGC9650.10174248502593115No Hit
GTCTGGGGCCCCAGGGCAGAGGATCACCATCTCCTGCACTGGGACCAGCT9630.1015316197719914No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9510.10026642824835288No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGATCACCATCTCCTGCACTGG9500.100160995621383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)