FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071008
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGTTCACCTCCCACTTTCGGCCCTGGGA111050.5362123178664688No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69110.3337022358194657No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65060.31414654120119284No Hit
GTATGGTAGTTCACCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT57920.27967057587416366No Hit
CAGTAATACACTGCAAAAGCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA56660.2735865819929233No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTCACCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA55430.26764744510885524No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43930.21211892952610517No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42170.2036206523586582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41170.19879208578624513No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39810.19222523524776342No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39560.19101809360466013No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39010.18836238198983296No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36890.1781258208563173No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36490.17619439422735209No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA35160.16977240068604274No Hit
GTAATACACTGCAAAAGCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33940.1638815494676988No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAAGCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC33270.16064640986418208No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33180.1602118388726649No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33040.1595358395525271No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32790.1583286979094238No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAACCTGAAGCTT30370.14664356680418425No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29470.1422978568890125No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29230.14113900091163337No Hit
GTAGTACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA29030.14017328759715075No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC27980.13510329269611707No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA26930.13003329779508335No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT26820.12950215547211794No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG26490.12790872850322163No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25510.12317673326225684No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT25290.12211444861632596No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24980.12061759297887792No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC24910.120279593318809No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT24840.11994159365874009No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24760.11955530833294706No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24720.11936216567005054No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA24630.11892759467853335No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG24430.11796188136405075No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24140.11656159705805097No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23250.11226417280860335No Hit
GACTTCACTCTCACCATCAGCAGACTGGAACCTGAAGCTTTTGCAGTGTATTACTGTCAGCAGTATGGTAGTTCA23100.11153988782274138No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22600.10912560453653487No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22340.10787017722770748No Hit
ACCACATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCCTGTATCTG21810.10531103694432856No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21680.10468332328991486No Hit
TGATTATACCATGCACTGGGTCCGTCAAACTCCGGGGAAGGGTCTGGAGT21660.10458675195846662No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21540.10400732396977704No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGC21370.10318646765246683No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC21140.10207589734081181No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA20970.1012550410235016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC453.4966433E-852.700333
TCGTATG3600.048.86925540
TATCGCG1650.047.77752336
CGTATGC4250.046.33349241
GTATCGC1700.046.3112135
ACCGATC8600.046.19463327
ATGCCGT3850.045.99898544
CCGATCA8800.045.54823728
AGACCGA8900.045.3533525
TATCTCG5000.045.24323336
CGCGTAT851.8189894E-1244.56949239
GACCGAT9100.044.3812526
CGATCAG9150.044.20501329
CACGAGA9250.043.97315221
ATCTCGT4750.043.34684437
CTTATAC9700.042.9742241
ACGAGAC9550.042.5979722
TCAGTAT9400.042.46251332
ATCTCCG9600.042.34950310
CGAGACC9650.042.1616323
Produced by FastQC (version 0.11.5)