FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670014
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64320.38514647182598466No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57580.34478752872730406No Hit
GAGTTACAGTACCCCTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT54540.32658408851662324No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42930.25706371323833216No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41610.24915958788369438No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40840.24454884809348904No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35030.20975872058557593No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34540.20682461344635433No Hit
CTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC33000.19760313386594364No Hit
GTAGGAGACAGAGTCACCATCGCTTGCCGGGCAAGTCAGAACATTATGAACTATTTAAATTGGTATCAGCAGAAA32930.1971839757031977No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32690.19574686200235447No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32180.19269299538806262No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32130.19239359670038694No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31860.19077684378693832No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31860.19077684378693832No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31510.1886810529732086No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30380.18191464263173843No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29630.17742366231660334No Hit
CCTCATGTATGGTACATCCAGTTTGCAAAGCGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTT27760.1662261513975332No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27230.16305252530817105No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTACTTTCGGCGGAGGGAC27210.16293276583310082No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC27190.16281300635803053No Hit
GTACATCCAGTTTGCAAAGCGGGGTCCCATCAAGGTTCAGTGGCAGTGGA26830.1606573358067657No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26570.1591004626308522No Hit
CCCCTAAGCTCCTCATGTATGGTACATCCAGTTTGCAAAGCGGGGTCCCA26070.15610647575409548No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGCCATCCAGATGACCCAGTC26020.1558070770664198No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTCATA25250.15119633727621445No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25070.15011850200058202No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTACTACTGTCAACAGAGTTACAGTACC24580.14718439486136042No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24450.1464059582734037No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23380.1399988263571443No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23300.13951978845686325No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22890.1370647192179227No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGCCAT22770.13634616236750113No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22650.1356276055170795No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22540.13496892840419303No Hit
GTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA22480.13460964997898223No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22260.13329229575320925No Hit
GTCTCCAGCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGGGCAAGTCAGAACATTATGAA22220.1330527768030687No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21850.13083722651426874No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGGGCAAGTCAGA20470.12257382273442019No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20220.12107682929604184No Hit
CTGGATGTACCATACATGAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTG20130.12053791165822561No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGCCATCCAGATGACCCAGTCTCCAGCCTCC20120.12047803192069048No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCG19950.1194600763825932No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19620.11748404504393377No Hit
GTGCCAGATGTGCCATCCAGATGACCCAGTCTCCAGCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCG19570.1171846463562581No Hit
AGTCAGAACATTATGAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA19310.1156277731803446No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19310.1156277731803446No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18960.11353198236661488No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18360.10993919811450684No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18340.10981943863943655No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17940.10742424913803118No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17900.10718473018789063No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17850.10688533150021497No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTCATG17780.10646617333746904No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17400.10419074331113393No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG17350.10389134462345825No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17170.10281350934782582No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTA17100.10239435118507988No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT17080.10227459171000962No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTACTACTGTCAACAGAGTTACAGTACCCCTACTTTCGG17040.10203507275986909No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17010.10185543354726367No Hit
GTCTGCAACCTGAAGATTTTGCGACTTACTACTGTCAACAGAGTTACAGT16990.10173567407219342No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16860.10095723748423666No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16730.1001788008962799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT150.002565326267.532531
TACGTAT702.1737396E-549.65662470
CGCGTAT405.0811286E-543.0118439
GCGAATA702.4590918E-838.9140933
GCGTATG557.4304808E-637.61108440
GGCGAAT6250.037.5479132
TAAGGCG6800.036.87045329
CGTATGC4050.036.6835741
GTGGTAT3600.036.5801241
CTTATAC7150.035.891421
ATGCCGT3700.035.69578644
GCGAATC5300.035.3347333
ACGAGAC6600.035.3074422
TCGTATG4000.034.47683340
ATATCGT400.002687512234.25887737
CGAATCT4700.034.0992734
CGAATAT501.994059E-434.0992734
CGAGACT6850.034.02088523
TCTCCGA7450.033.97558611
AGGCGAA7200.033.9677431
Produced by FastQC (version 0.11.5)