FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670014
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68010.4072420949764493No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63240.3786794601721902No Hit
GAGTTACAGTACCCCTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT53240.31879972263705575No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47400.2838299559165372No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43290.25921938378959697No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42900.2568840740257267No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36990.2214951491424623No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35020.2096988408480408No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34570.20700425265895972No Hit
GTAGGAGACAGAGTCACCATCGCTTGCCGGGCAAGTCAGAACATTATGAACTATTTAAATTGGTATCAGCAGAAA33020.19772289334101392No Hit
CTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC32460.1943696280390464No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31550.18892057192334916No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31100.18622598373426807No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30220.18095656683117628No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29810.17850149759223577No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29430.17622606756590065No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27730.1660465121849278No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27570.16508843638436563No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26780.16035793711909002No Hit
GTACATCCAGTTTGCAAAGCGGGGTCCCATCAAGGTTCAGTGGCAGTGGA26430.1582621463053603No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26330.15766334893000897No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC26250.1571843110297279No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26020.1558070770664198No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25940.15532803916613874No Hit
CCCCTAAGCTCCTCATGTATGGTACATCCAGTTTGCAAAGCGGGGTCCCA24950.14939994515016042No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24930.14928018567509016No Hit
CCTCATGTATGGTACATCCAGTTTGCAAAGCGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTT24760.14826223013699286No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24530.14688499617368478No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGCCATCCAGATGACCCAGTC24370.1459269203731226No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTCATA24310.1455676419479118No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTACTTTCGGCGGAGGGAC24160.14466944588488478No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24100.14431016745967398No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23580.141196421107847No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTACTACTGTCAACAGAGTTACAGTACC23540.14095690215770645No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23460.14047786425742537No Hit
GTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA22590.1352683270918687No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGGGCAAGTCAGA21920.1312563846770147No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20950.12544805013610666No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20570.12317262010977154No Hit
GTCTCCAGCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGGGCAAGTCAGAACATTATGAA20500.12275346194702559No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGCCAT20350.12185526588399857No Hit
CTGGATGTACCATACATGAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTG20250.12125646850864723No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20220.12107682929604184No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGCCATCCAGATGACCCAGTCTCCAGCCTCC20090.12029839270808508No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19990.11969959533273374No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19780.11844212084449592No Hit
GTGCCAGATGTGCCATCCAGATGACCCAGTCTCCAGCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCG19590.11730440583132835No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19180.11484933659238784No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCG19150.11466969737978244No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18700.11197510919070139No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18550.11107691312767437No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18420.11029847653971764No Hit
AGTCAGAACATTATGAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA18110.10844220467612846No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTCATG18110.10844220467612846No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17990.10772364782570686No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17440.10443026226127446No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17000.10179555380972853No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16980.10167579433465827No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16970.10161591459712314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3650.043.3687931
GTATCAA33200.031.549661
TATCAAC36350.028.4407652
ATCAACG36550.028.1025563
TCAACGC36500.027.9556854
CAACGCA37000.027.5779025
AACGCAG38000.026.9407886
AACGGCC1800.026.6262937
TAACGGC1707.2759576E-1226.13740536
ACGCAGA40650.025.1845027
GCGGTAC1801.0913936E-1125.121444
CGCAGAG41200.024.7665638
CGGTACC1851.6370905E-1124.49018345
AGAGTAC42300.024.43876811
CGCGGTA1851.6370905E-1124.39188643
TTAACGG1852.1827873E-1123.9831635
GACAGGT3150.022.4533481
AAGCGAT18050.022.20056770
GGCACCG2007.2759576E-1121.8893939
TAACCGT1956.566552E-1021.83760653
Produced by FastQC (version 0.11.5)