FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366839
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48170.35241897546089923No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40620.297182038264931No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38700.2831350290707245No Hit
GTAATATATAGCCGTGTCTTCGTCTCTCAGGCTGTTCATTTGCAAATACA38290.2801354073157116No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35280.2581137939435442No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35150.25716269436268646No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34410.2517487429024194No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTCTCCTGGTATCAGCAA28170.2060959630212483No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27150.1986334893868261No Hit
ATATATTACTGTGCGAGGGAGAATATTAGGATGTTTAAGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGT26800.1960728366691322No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26240.191975792320822No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT26160.19139050027106339No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25970.1900004316528867No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC25890.18941513960312809No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG25670.18780558646629195No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24610.18005046680699044No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24180.17690452203953794No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC24160.1767581990270983No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCTCACTCGGGCTGCTATCCCATGTT24140.17661187601465864No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCTCACTCGGG23320.17061263250463296No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCCGAGTGAGGGAGTCTTCGG22180.16227222079557285No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21940.16051634464629705No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG20720.15159064088747834No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG20610.15078586431906024No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCTCACTCGGGC20460.14968844172576287No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19340.14149435302914243No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG19150.14010428441096573No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18470.1351293019880176No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18250.13351974885118145No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17980.13154438818324615No Hit
AGTATATTCTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATTTG17970.13147122667702635No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17790.13015431956506948No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17570.12854476642823331No Hit
GTTTGGGGGCTGTTCCTGGGAGTTGCTGATACCAGGAGACATAATTATTC17560.12847160492201348No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16670.12196023086844902No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16490.12064332375649217No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC16300.11925325513831549No Hit
GCCCCCAAACTCCTCATTTATGACAATGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT16180.11837531706367758No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTCTCCTGGTATCAGCAACTCCCAGGAACAGCCCC15770.11537569530866473No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAA15500.11340033464072945No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15110.11054703589815625No Hit
GTTCCTGGGAGTTGCTGATACCAGGAGACATAATTATTCCCAATGTTGGA15050.11010806686083731No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT15020.10988858234217783No Hit
GGATAGCAGCCCGAGTGAGGGAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC14920.10915696727997959No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCC14810.10835219071156149No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTCGGAAGATATACCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG14520.10623050703118657No Hit
GTTCCGAAGACTCCCTCACTCGGGCTGCTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGT14220.10403566184459179No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14180.10374301581971249No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTACTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT14020.10257243172019528No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13940.10198713967043667No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13940.10198713967043667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)