FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366839
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74380.5441752832630617No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43010.31466763825146926No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40830.2987184298955473No Hit
GTAATATATAGCCGTGTCTTCGTCTCTCAGGCTGTTCATTTGCAAATACA36650.2681369202956603No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34470.2521877119397383No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33740.24684692198569108No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG30590.22380104752644606No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29960.21919187263459705No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29640.21685070443556265No Hit
ATATATTACTGTGCGAGGGAGAATATTAGGATGTTTAAGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGT28810.21077829941931714No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC27270.19951142746146402No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26870.19658496721267096No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTCTCCTGGTATCAGCAA26130.1911710157524039No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23990.17551445342136124No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23370.1709784400357321No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23260.170173663467314No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23080.16885675635535713No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCTCACTCGGGCTGCTATCCCATGTT22790.1667350726749822No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22130.16190641326447375No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCCGAGTGAGGGAGTCTTCGG21970.16073582916495652No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG21880.16007737560897808No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21770.15927259904056001No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC21270.15561452372956874No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20280.148371534613806No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG19990.1462498509334311No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG18880.13812892374303046No Hit
AGTATATTCTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATTTG17720.12964218902153068No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17680.12934954299665138No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17480.1278863128722549No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG17290.1264962442540782No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCTCACTCGGG17240.12613043672297908No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17080.12495985262346188No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17010.1244477220799231No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16560.12115545430003095No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCCTCACTCGGGC16260.1189606091134362No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCC15940.11661944091440177No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15850.11596098735842333No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT15800.11559517982732422No Hit
GGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC15420.11281504259097085No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT15300.11193710451633293No Hit
GGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT15170.11098600493547521No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14750.10791322167424254No Hit
GTTTGGGGGCTGTTCCTGGGAGTTGCTGATACCAGGAGACATAATTATTC14690.10747425263692358No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14650.10718160661204429No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT14650.10718160661204429No Hit
GCCCCCAAACTCCTCATTTATGACAATGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT14320.10476727690679004No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC14170.10366985431349268No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTCGGAAGATATACCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG14040.10271875473263493No Hit
GGATAGCAGCCCGAGTGAGGGAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC13910.10176765515177721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGA2350.040.5666570
GTATCAA36450.038.2736471
CGGGACG451.0885036E-437.795834
GTGGTAT4050.033.606131
TATCAAC41900.032.806882
ATCAACG42550.032.46093
TCAACGC43050.031.9223334
CAACGCA43650.031.4835365
AACGCAG43900.031.3042456
GACAGGT3000.030.6235871
CGCAGAG45600.029.9134128
GATCTGG3201.8189894E-1229.79113270
AGAGTAC46350.029.6462911
GAGTTTA3450.029.5880051
CAGAGTA46800.029.14533410
GCAGAGT47400.028.777469
GTGGTGA3600.028.51800770
AGTTTAT3850.028.2804892
TATACTT500.00828713327.2129975
TCTCGCG6800.026.960370
Produced by FastQC (version 0.11.5)