FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647207
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20800.3213809492171747No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC14250.22017685222811248No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12690.1960732810368244No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12540.193755629960739No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12290.18989287816726333No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12180.18819326737813405No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11580.17892266307379245No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10470.16177204511076054No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10160.15698223288685073No Hit
GCCTTGGGCTGACGAAGGACGGTCAGCTTGGTTCCTCCGCCGAACACCCAGGGATTCCTGCCGCCTGCAAATGAG10150.1568277228151117No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT9960.1538920314520702No Hit
GTCACTCACCATCCCCTGCACTGGAACCAGCAGTGACGTCGGTGGTTATGACCATGTCTCCTGGTACCAACAACA9780.15111085016076772No Hit
GATTATTACTGCAGCTCATTTGCAGGCGGCAGGAATCCCTGGGTGTTCGGCGGAGGAACCAAGCTGACCGTCCTT9660.14925672929989942No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9570.1478661386542482No Hit
GTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG9490.14663005808033597No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGAAGGACGGTCAGCTTGGTTCCTC9030.13952259478034076No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8860.13689592356077732No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8820.1362778832738212No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8730.13488729262816995No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8180.12638923868252352No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8130.12561668832382838No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8090.12499864803687229No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7970.12314452717600397No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7970.12314452717600397No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7930.12252648688904787No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7920.12237197681730884No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC7740.11959079552600638No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA7650.11820020488035513No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7580.11711863437818194No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATTT7550.11665510416296486No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCTGGGTCTCTTGGACAGTCACTCACCATCCCCTG7340.1134103926564453No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7290.11263784229775017No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7180.1109382315086209No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7040.10877509050427452No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6940.10722998978688425No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC6650.1027491977064525No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC6600.10197664734775737No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCTGGGTCTCTTGGACAGTCACTCAC6520.10074056677384516No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGAAGGACGGTCAGCTTGGTTCCTCCGCCGAACAC6510.10058605670210613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG150.002612083767.2208416
GTGGTAT2850.041.2536161
TAGGATA350.00121811240.2454949
TGGTATC3000.039.2000272
GGTATCA7750.038.1434331
CTGTACG400.002599844234.48809441
GTATCAA16500.034.406591
GACAGGT1156.366463E-1132.13171
CAGCGAT450.004748773830.49632839
ACAGCGA450.00481887930.40510238
ACTGATA450.00526485129.8597613
AACGCAG20000.028.396416
ATCAACG19900.028.3658493
CAACGCA20300.027.8112165
TCAACGC20250.027.7160784
GTACGAA500.007575813727.71586643
ACGCAGA20700.027.4361447
CGCAGAG20850.027.2387628
GAAGTGT500.00878998626.8841766
GAATAAG500.00879661926.88001614
Produced by FastQC (version 0.11.5)