FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235150
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACAGTATCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT46950.3801157754118933No Hit
GTCTTCAGTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCCACTTACTACTGTCAACAGAGTTACAGTATC46800.37890134801441117No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45730.370238432579039No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41340.3346961907460632No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG31600.2558393717362264No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31000.250981662146298No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30500.24693357082135772No Hit
CTGTAACTCTGTTGACAGTAGTAAGTGGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGACTGAAGACTGTC29880.24191393757843177No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29100.2355989151115249No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27020.21875885519977328No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26060.21098651985588796No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25570.20701939035744646No Hit
GTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATTTGGGACAGT25200.20402380277699064No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25020.20256648990001214No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24230.19617050560660648No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTCCCGCTGATACCAATTTAAAT23740.192203376108165No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTCCCGCTGATACCAATT23420.18961259766020322No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACACGTATTTAAATTGGTATCAGCGGGAA23020.18637412460025096No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT22820.18475488807027488No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22480.18200218596931547No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22470.18192122414281667No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT22310.18062583491883577No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21820.17665870542039427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21660.17536331619641338No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21280.17228676678945876No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20900.16921021738250414No Hit
CCTTAGGGGCTTTCCCTGGTTCCCGCTGATACCAATTTAAATACGTGTTA20690.16751001902602922No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGGATA19590.1586042181111606No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCACCTTCAGGGAGA19300.15625632514269522No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18120.14670282961583614No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17580.14233089098490062No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17230.1394972270574424No Hit
GACTGAAGACTGTCCCAAATCCACTGCCACTGAACCTTGATGGGACCCCA17010.1377160668744687No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16880.13666356312998423No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16860.1365016394769866No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16750.13561105938549972No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16690.1351252884265069No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14630.1184471521677529No Hit
GTAGTAAGTGGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGACTGAAGACTGTCCCAAATCCACTGCCA14420.11674695381127798No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14360.11626118285228515No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14240.11528964093429948No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14000.11334655709832814No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13960.1130227097923329No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13840.11205116787434725No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13730.11116058778286038No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13590.11002712221187709No Hit
ACTTACTACTGTCAACAGAGTTACAGTATCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13560.10978423673238069No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13380.10832692385540219No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13040.10557422175444277No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13010.10533133627494637No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12910.1045217180099583No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12880.1042788325304619No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12870.1041978707039631No Hit
GTCCCAAATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT12670.10257863417398695No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12450.10079747399101323No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12450.10079747399101323No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12440.10071651216451442No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12390.1003117030320204No Hit
GATTTGGGACAGTCTTCAGTCTCACCATCAGCAGTCTGCAACCTGAAGAT12370.10014977937902278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)