FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235150
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72370.5859207383718577No Hit
GTCTTCAGTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCCACTTACTACTGTCAACAGAGTTACAGTATC46920.3798728899323969No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46330.37509614216896736No Hit
GAGTTACAGTATCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT46320.37501518034246856No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34370.2782657976763956No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG30750.24895761648382786No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30110.2437760595879043No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29300.23721815164150103No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28790.23308909849006193No Hit
CTGTAACTCTGTTGACAGTAGTAAGTGGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGACTGAAGACTGTC28350.22952677812411448No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26850.2173825041492936No Hit
GTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATTTGGGACAGT25930.20993401611140347No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24560.1988422458810671No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24250.1963324292596041No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23850.19309395619965186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22880.1852406590292677No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACACGTATTTAAATTGGTATCAGCGGGAA22800.18459296441727724No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTCCCGCTGATACCAATTTAAAT22330.18078775857183338No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21160.1713152248714731No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT21130.17107233939197666No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTCCCGCTGATACCAATT21040.17034368295348745No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20320.1645144314455734No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19950.16151884386511758No Hit
CCTTAGGGGCTTTCCCTGGTTCCCGCTGATACCAATTTAAATACGTGTTA19850.16070922560012954No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19570.15844229445816296No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGGATA19030.15407035582722745No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18440.14929360806379793No Hit
GACTGAAGACTGTCCCAAATCCACTGCCACTGAACCTTGATGGGACCCCA18290.14807918066631584No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18100.1465409059628385No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17980.14556936404485285No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCACCTTCAGGGAGA17950.14532647856535644No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17730.1435453183823827No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17300.14006395984293404No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16510.13366797554952842No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16360.13245354815204632No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15540.12581467837914423No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15300.1238715945431729No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14840.12014735052422784No Hit
GTAGTAAGTGGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGACTGAAGACTGTCCCAAATCCACTGCCA14830.12006638869772902No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14680.11885196130024694No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14290.1156944500667935No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14100.11415617536331618No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14000.11334655709832814No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14000.11334655709832814No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13940.11286078613933531No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13550.10970327490588189No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13550.10970327490588189No Hit
ACTTACTACTGTCAACAGAGTTACAGTATCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13230.10711249645792009No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13090.10597903088693682No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATTTGGGACAGTCTTCAGT12850.10403594705096547No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12810.10371209974497024No Hit
GTCCCAAATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT12710.1029024814799822No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12680.10265959600048577No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12630.10225478686799175No Hit
AGTCAGAACATTAACACGTATTTAAATTGGTATCAGCGGGAACCAGGGAAAGCCCCTAAGGTCCTGATCTATG12420.10055458851151683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3200.049.698311
CTAACGC306.9488614E-445.1107143
GTATCAA36450.039.5462951
GACAGGT2750.036.9132541
TACGTAT800.002174873635.76560670
TATCAAC42300.034.1502042
TACTGAT8200.034.02094770
AATGGTA4850.033.9220270
AGGTACG502.088707E-433.83032
ATCAACG42600.033.5947763
AACGCAG42800.033.201996
TCAACGC42900.033.125944
CAACGCA43100.032.9708865
ATTAGAC450.003250309732.9543866
CGCAGAG44150.032.1101158
ACGCAGA44550.031.8218127
AGAGTAC45800.031.2424811
GCAGAGT46650.030.3893179
CCTAACG450.00508794530.0713732
AGTAACG450.00508794530.0713732
Produced by FastQC (version 0.11.5)