FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457016
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT53880.36979689996540877No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA51400.35277581028622884No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACAGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT46530.31935133176300057No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG44420.30486967885047245No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC43530.2987613039252829No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCATCGTCTCCTGTATTGGAACCAG39600.27178836745787277No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39430.27062159921373546No Hit
TCTCTGGGCTCCGGACTGAGGATGAGGCTGATTATTACTGCTGCTCATAT38960.2673958281858264No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38920.2671212944813235No Hit
GTATTGGAACCAGCAGTGATGTTGGTGCTTATGACTATGTCTCCTGGTAC36110.2478353017399946No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCTGTGTAGCTGCCTGCATATGAGCAGCAGTAATAAT33180.22772570788515706No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30530.2095378499618398No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29860.20493941041141622No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAT29580.20301767447989588No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29480.20233134021863863No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCTGTGTAGCTGCCTGCATATGAGCAG28700.19697793298083205No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC28630.196497498997952No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCTGTGTAG28450.19526209732768893No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27910.19155589231689976No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCATCGTCTC27660.1898400566637566No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC27460.18846738814124211No Hit
GTAATAATCAGCCTCATCCTCAGTCCGGAGCCCAGAGATGGTCAGGGAGG27400.18805558758448773No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG27160.18640838535747034No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCAGGCTCCAAGTCTGGCACCACGG25910.17782920709175465No Hit
CTCCTGGACAGTCAGTCATCGTCTCCTGTATTGGAACCAGCAGTGATGTT25710.17645653856924015No Hit
CCCATGGGGTGTCTTGTCACAGATACACCTGCAGCAGTCAGGTCCAGGAC25620.1758388377341086No Hit
CAATAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGCTGCAGGGA25000.17158356531431362No Hit
GTATTATTGTGTAAGGGGAACAGGTATGGGAACAGCCTCGAGGCACTACTTTGACTACTGGGGCCAGGGAACC24240.1663674249287585No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23340.1601904165774432No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCTGTGTAGCTGC23290.15984724944681458No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTACACAGAAGTCTTCGGAACTGGGACCAAGGTCAC23090.15847458092430008No Hit
ATAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA22550.1547683759135109No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22530.15463110906125946No Hit
GTGCCAGACTTGGAGCCTGAGAAGCGATCAGGGACCCCTGAGGGCCGCTC22450.15408204165225364No Hit
GTCTGGCACCACGGCCTCCCTGACCATCTCTGGGCTCCGGACTGAGGATG22250.15270937312973915No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22120.1518171385901047No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC22060.15140533803335035No Hit
ACACAGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC21630.14845410070994414No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20870.14323796032438904No Hit
CCCCTGAGGGCCGCTCACTGACATCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA20790.14268889291538323No Hit
CTTCTGTGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCC20710.14213982550637744No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20280.13918858818297122No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20260.1390513213307198No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20080.13781591966045673No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19740.13548238317218206No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT19660.13493331576317624No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCATCGTCT19480.1336979140929132No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19430.13335474696228455No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19180.13163891130914143No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCATCGTCTCCTGTATTGGAACCAGCAGTGATGTTGGTG18990.13033487621275264No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18350.12594233694070622No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGGTGCCAGACTTGGAGCCTGAGAAGCGATCAGGGACCCCTGAGGGCCGCT17950.1231969998956772No Hit
CCTGAGGGCCGCTCACTGACATCATAAATTATGAGTTTGGGGGCTTTGCC17160.11777495923174489No Hit
GTAGTATGCCCTTCCCAGCCACTCAAGGCCTCTCGATGGGGACTGCCTGA16980.11653955756148182No Hit
CTACAGGTCCAAGTGGTATAATGATTATGCAGTTTCTGTGAAGAGTCGAA16880.11585322330022456No Hit
GCTTATGACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCAGTGAG16810.11537278931734449No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16580.1137942205164528No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAT16550.11358832023807564No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16530.11345105338582417No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16430.11276471912456693No Hit
GTCAGTCATCGTCTCCTGTATTGGAACCAGCAGTGATGTTGGTGCTTATGACTATGTCTCCTGGTACCAACAGCA16250.11152931745430388No Hit
GTCCAGGACTGGTGAAGCCCTCGCAGACCCTCTCAGTCACCTGTGCCATC16080.11036254921016654No Hit
GTGTATTATTGTGTAAGGGGAACAGGTATGGGAACAGCCTCGAGGCACTACTTTGACTACTGGGGCCAGGGAACC15920.10926441439215492No Hit
CCTCAGGGGTCCCTGATCGCTTCTCAGGCTCCAAGTCTGGCACCACGGCCTCCCTGACCATCTCTGGGCTCCGGA15630.1072740450345089No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT15440.1059700099381201No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15300.10500914197235994No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15230.10452870798947987No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT15200.1043228077111027No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15110.10370510687597116No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15070.10343057317146827No Hit
GTCCGGAGCCCAGAGATGGTCAGGGAGGCCGTGGTGCCAGACTTGGAGCC15070.10343057317146827No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15060.10336193974534254No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15010.1030187726147139No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14780.10144020381382221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG4400.043.64779727
CGTATGC4200.043.5714841
TCGTATG4150.043.23004540
ATGCCGT4350.042.95338444
GGTATCA12750.042.760161
ATGCGCA4500.041.9431129
TCTCGTA3700.040.97287838
TATACAC4600.040.9665373
CCGTCTT4600.040.70359847
TGCGCAG4650.040.60685730
CATGCGC4650.040.57476828
AGGGGGG4500.040.4109170
ATCTCGT3600.040.17746437
CTTATAC4700.040.1252021
ACGAGAC4700.040.10591522
GTATCAA30550.039.7323651
CACGAGA4750.039.67830321
CTTGAAA4900.039.3496257
TTATACA4850.038.8548552
CGAGACA4950.038.77273623
Produced by FastQC (version 0.11.5)