FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457016
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC48920.3357547206070489No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47700.3273814426197104No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA46570.3196258654675035No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45650.3133115902639367No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43640.29951627161266586No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACAGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT43280.29704546827213973No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG40340.2768672409911765No Hit
TCTCTGGGCTCCGGACTGAGGATGAGGCTGATTATTACTGCTGCTCATAT36970.253737776386807No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCATCGTCTCCTGTATTGGAACCAG36560.2509238059156523No Hit
GTATTGGAACCAGCAGTGATGTTGGTGCTTATGACTATGTCTCCTGGTAC36050.24742350118324027No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCTGTGTAGCTGCCTGCATATGAGCAGCAGTAATAAT31860.2186660956365613No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31370.21530305775640077No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAT30930.21228318700686885No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCTGTGTAG29370.20157637253125568No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCTGTGTAGCTGCCTGCATATGAGCAG28970.19883103548622663No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28010.19224222657815698No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27850.1911440917601454No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG27810.19086955805564249No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27570.18922235582862507No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27390.187986954158362No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCATCGTCTC27050.18565341767008736No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26980.18517298368720728No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26980.18517298368720728No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24810.17027953021792486No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24190.16602425779812988No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC24180.16595562437200415No Hit
GTATTATTGTGTAAGGGGAACAGGTATGGGAACAGCCTCGAGGCACTACTTTGACTACTGGGGCCAGGGAACC24060.16513202325849546No Hit
CTCCTGGACAGTCAGTCATCGTCTCCTGTATTGGAACCAGCAGTGATGTT24010.1647888561278668No Hit
GTAATAATCAGCCTCATCCTCAGTCCGGAGCCCAGAGATGGTCAGGGAGG23950.16437705557111246No Hit
CCCATGGGGTGTCTTGTCACAGATACACCTGCAGCAGTCAGGTCCAGGAC23890.16396525501435813No Hit
CAATAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGCTGCAGGGA22650.15545471017476817No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCAGGCTCCAAGTCTGGCACCACGG22520.15456247563513373No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTACACAGAAGTCTTCGGAACTGGGACCAAGGTCAC22490.15435657535675656No Hit
GTGCCAGACTTGGAGCCTGAGAAGCGATCAGGGACCCCTGAGGGCCGCTC22350.1533957073909964No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22280.15291527340811634No Hit
GTCTGGCACCACGGCCTCCCTGACCATCTCTGGGCTCCGGACTGAGGATG21330.1463950979261724No Hit
CTTCTGTGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCC21160.14522832968203506No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21100.14481652912528073No Hit
ATAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA20960.14385566115952056No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTCTGTGTAGCTGC20550.1410416906883658No Hit
ACACAGAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC20220.13877678762621687No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19960.13699231854694802No Hit
CCCCTGAGGGCCGCTCACTGACATCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA19600.1345215152064219No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19560.134246981501919No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19470.13362928066678748No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18420.12642277092358628No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCATCGTCTCCTGTATTGGAACCAGCAGTGATGTTGGTG17990.1234715336001801No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17820.12230476535604276No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCATCGTCT17780.12203023165153987No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17670.12127526396415687No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAT17510.12017712914614527No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17460.11983396201551665No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGGTGCCAGACTTGGAGCCTGAGAAGCGATCAGGGACCCCTGAGGGCCGCT17420.11955942831101375No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17210.11811812636237351No Hit
GTGTATTATTGTGTAAGGGGAACAGGTATGGGAACAGCCTCGAGGCACTACTTTGACTACTGGGGCCAGGGAACC16350.11221565171556111No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16130.11070571634079515No Hit
GCTTATGACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCAGTGAG16120.11063708291466944No Hit
GTCAGTCATCGTCTCCTGTATTGGAACCAGCAGTGATGTTGGTGCTTATGACTATGTCTCCTGGTACCAACAGCA15840.10871534698314912No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15530.10658771077325163No Hit
CCTGAGGGCCGCTCACTGACATCATAAATTATGAGTTTGGGGGCTTTGCC15430.10590137651199437No Hit
GTAGTATGCCCTTCCCAGCCACTCAAGGCCTCTCGATGGGGACTGCCTGA15260.10473460826785704No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15230.10452870798947987No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT15230.10452870798947987No Hit
GTCCAGGACTGGTGAAGCCCTCGCAGACCCTCTCAGTCACCTGTGCCATC15160.1040482740065998No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15090.1035678400237197No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14990.10288150576246247No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT14990.10288150576246247No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14700.10089113640481641No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14670.10068523612643925No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14580.10006753529130771No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGACG400.005863775554.80656470
CGGCTCG400.005863775554.80656470
AGTATCG200.007193320452.04962548
GGTATCA12400.046.0621
TGGTATC3300.041.4552082
GTATCAA29950.037.9130971
AATGGTA3700.037.5252170
CGCAGTC400.002664638534.31838232
TATCAAC34600.032.421332
GACAGCC3150.031.4872991
ATCAACG36000.031.4455053
TCAACGC36550.031.1583924
CAACGCA36950.030.8179135
AACGCAG37250.030.6615186
GACAGGT2800.030.5372541
TAACGCT450.00482529130.3994454
GCTTGCC704.08678E-529.3157631
AAAACCG1000.005843014529.23016570
CGGGTAC953.4495315E-728.80936417
ACGCAGA39600.028.7556047
Produced by FastQC (version 0.11.5)