FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454371
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44950.9892796855433116No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12960.28522947107099705No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10330.22734725587680552No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10070.22162505969791205No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9280.2042383866928127No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8480.18663162921929438No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8360.1839906155982666No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6450.1419544821302416No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC6190.13623228595134812No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG5990.13183059658296853No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA5980.13161051211454958No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC5850.12874941402510284No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC5790.12742890721458897No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT5690.12522806253039917No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5680.1250079780619802No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC5630.12390755571988529No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG5520.12148662656727652No Hit
GCTTATTACTGTGCAGCGTGGGATGACAGCCTGAATGGTCGGGTGTTCGG4930.10850164293055675No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT4860.1069610516516239No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC4820.10608071377794799No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC4740.10432003803059614No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG4660.10255936228324432No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT4640.10211919334640635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCGA150.00258942367.36545623
CGGTCTA200.007201476552.02867542
CGGCCTA200.00795069350.7425229
GGTATCA13200.040.2372631
GTATCAA30400.036.3804051
CCGGCGT400.002415052435.00808747
CCGCTAT1301.8989735E-532.7776670
ACAGGTA1101.3933459E-930.5599461
GTGGTAT3300.030.5599461
TATAGAG553.793667E-430.5532155
AACGCAG36150.030.4940516
ACCCGCG450.004821398830.39988936
ATCAACG36700.030.1286323
AACGGGT450.00511639730.0330228
TCAACGG450.00516087429.97990826
TCAACGC36800.029.9554354
TATCAAC37250.029.6000752
CAACGCA37300.029.5538885
CGCAGAG37850.029.390828
ACGCAGA38450.028.8447767
Produced by FastQC (version 0.11.5)