FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429263
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56370.3943990714095307No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49520.3464722727727507No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38630.2702791578596801No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38570.26985936108329955No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38500.26936959817752226No Hit
GTCCGAGTGTTACTGGTTCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCTTGATCTATAAGT34420.24082341738364457No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG32710.22885920925679878No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32130.2248011737517868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30480.21325676240132155No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29330.2052106575206942No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCCGAGTGTTACTGGTTCGTTGGCCTGGTATCAGCAGAAA29170.20409119945034607No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28730.20101268975688869No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28460.19912360426317618No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28330.19821404458101832No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCCGA28310.19807411232222483No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27940.1954853655345447No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27880.19506556875816417No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27870.19499560262876742No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26710.18687953161874335No Hit
CTACTGATGGTGAGAGTGGATTCTGTCCCAGATCCACTGCCGCTGAATCT26560.185830039677792No Hit
CCATAGTGTTGGCAGTAATAAGTTGCAGAATCGTCAGGCTGCAGACTACT25770.18030271545544801No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25590.17904332512630636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25330.17722420576199063No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACGAACCAGTA25080.17547505252707166No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22960.16064223309495873No Hit
GGAATAAACACCATAGTGTTGGCAGTAATAAGTTGCAGAATCGTCAGGCT22510.15749375727210457No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACAT22450.157073960495724No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACATCCAGATGACCCAGTCTCCTTCCACC21960.15364562015528282No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21870.15301592499071198No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21450.15007734755604812No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAATCTGGTCCCGGGGCCGAAAGTG21430.1499374152972546No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21090.1475585668977648No Hit
ACTTATTACTGCCAACACTATGGTGTTTATTCCCGAGTATTCACTTTCGGCCCCGGGACCAGATTGGAGATCAAA21030.14713877012138424No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT21010.14699883786259074No Hit
GAATCCACTCTCACCATCAGTAGTCTGCAGCCTGACGATTCTGCAACTTA20970.1467189733450037No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20770.1453196507570685No Hit
GTTTATTCCCGAGTATTCACTTTCGGCCCCGGGACCAGATTGGAGATCAAACGAACTGTGGCTGCACCAT20760.14524968462767174No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20650.1444800572043074No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20610.14420019268672038No Hit
CTTATAGATCAAGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACGAACCAGTAACACTCGGA20180.14119164912265972No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20060.1403520555698986No Hit
CCCCTAAGGTCTTGATCTATAAGTCGTCGACTTTAGAAAGTGGGGTCCCA19970.1397223604053278No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19890.13916263137015372No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19770.1383230378173926No Hit
GTCTTGATCTATAAGTCGTCGACTTTAGAAAGTGGGGTCCCATCAAGATT19510.13650391845307686No Hit
GAGTGGATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA19510.13650391845307686No Hit
ATCTTGATGGGACCCCACTTTCTAAAGTCGACGACTTATAGATCAAGACCTTAGGGGCTTTCCCTGGTTTCTG19200.13433496844177734No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAATCTGGTCC18520.12957727164279773No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCTTGATCTATAAGTCGTCGACTTTAGAAAGTGGGGTCCCAT18480.12929740712521068No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18300.12803801679606902No Hit
ACACTATGGTGTTTATTCCCGAGTATTCACTTTCGGCCCCGGGACCAGAT18150.12698852485511763No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18040.1262188974317533No Hit
GTAATAAGTTGCAGAATCGTCAGGCTGCAGACTACTGATGGTGAGAGTGGATTCTGTCCCAGATCCACTGCCG17880.12509943936140513No Hit
CACTCGGACTGGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGATGCA17680.12370011677346995No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17470.12223082805613802No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATCCACTCTCACCATCAGTAGT17210.12041170869182229No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16880.11810282642172923No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16680.11670350383379405No Hit
GTAATAAATAGCCGTGTCTGCGGCGGTCACAGAGCCCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCAGCGGA16560.11586391028103295No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16430.1149543505988751No Hit
CATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATCCACTCTCACCATCAGTAGTCTGCAGCCTGACGATTCTG16360.11446458769309777No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16290.11397482478732045No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16020.11208573929360796No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15900.11124614574084686No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15830.11075638283506953No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15670.1096369247647214No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15660.10956695863532465No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15650.10949699250592788No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15400.10774783927100891No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15160.10606865216548668No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14880.10410960054237743No Hit
ATCTGGGACAGAATCCACTCTCACCATCAGTAGTCTGCAGCCTGACGATT14800.10354987150720336No Hit
CCATCAGTAGTCTGCAGCCTGACGATTCTGCAACTTATTACTGCCAACACTATGGTGTTTATTCCCGAGTATTCA14750.10320004086021957No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14720.1029901424720293No Hit
CATCAGTAGTCTGCAGCCTGACGATTCTGCAACTTATTACTGCCAACACTATGGTGTTTATTCCCGAGTATTCA14670.10264031182504549No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14580.10201061666047467No Hit
ATCTATAAGTCGTCGACTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATCCACT14390.10068126020193624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)