FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429263
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55590.38894171331658345No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41640.2913389628081046No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39150.2739173965883116No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38560.2697893949539028No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37220.2604139336147371No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35400.24768009806452698No Hit
GTCCGAGTGTTACTGGTTCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCTTGATCTATAAGT34710.2428524351361506No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG30700.2147960172480502No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30290.21192740594278311No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30150.21094788013122848No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCCGAGTGTTACTGGTTCGTTGGCCTGGTATCAGCAGAAA29450.20605025107345531No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29170.20409119945034607No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCCGA29110.20367140267396555No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28940.20248197847422064No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28010.19597512844032203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26460.1851303783838244No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26310.184080886442873No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26140.1828914622431281No Hit
CTACTGATGGTGAGAGTGGATTCTGTCCCAGATCCACTGCCGCTGAATCT25150.17596481543284895No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24820.1736559331627559No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23490.16435043795298696No Hit
CCATAGTGTTGGCAGTAATAAGTTGCAGAATCGTCAGGCTGCAGACTACT23430.1639306411766064No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23330.1632309798826388No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACGAACCAGTA23320.16316101375324205No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23270.16281118310625828No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23100.16162175890651337No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22270.15581457016658237No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21830.15273606047312496No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACATCCAGATGACCCAGTCTCCTTCCACC21820.15266609434372821No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAATCTGGTCCCGGGGCCGAAAGTG21540.15070704272061897No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21460.15014731368544487No Hit
GGAATAAACACCATAGTGTTGGCAGTAATAAGTTGCAGAATCGTCAGGCT21250.14867802496811294No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21070.14741863463897126No Hit
GAATCCACTCTCACCATCAGTAGTCTGCAGCCTGACGATTCTGCAACTTA20860.14594934592163933No Hit
CTTATAGATCAAGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACGAACCAGTAACACTCGGA20330.1422411410636111No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACAT20190.14126161525205647No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20130.14084181847567592No Hit
ACTTATTACTGCCAACACTATGGTGTTTATTCCCGAGTATTCACTTTCGGCCCCGGGACCAGATTGGAGATCAAA20060.1403520555698986No Hit
GAGTGGATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA19940.1395124620171375No Hit
GTTTATTCCCGAGTATTCACTTTCGGCCCCGGGACCAGATTGGAGATCAAACGAACTGTGGCTGCACCAT19880.13909266524075697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19520.13657388458247363No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19440.13601415554729956No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19150.13398513779479354No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19080.13349537488901622No Hit
GTCTTGATCTATAAGTCGTCGACTTTAGAAAGTGGGGTCCCATCAAGATT18850.13188615391289077No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCTTGATCTATAAGTCGTCGACTTTAGAAAGTGGGGTCCCAT18720.1309765942307329No Hit
CCCCTAAGGTCTTGATCTATAAGTCGTCGACTTTAGAAAGTGGGGTCCCA18280.1278980845372755No Hit
ATCTTGATGGGACCCCACTTTCTAAAGTCGACGACTTATAGATCAAGACCTTAGGGGCTTTCCCTGGTTTCTG18260.12775815227848197No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18210.12740832163149818No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18040.1262188974317533No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17970.12572913452597598No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17600.12314038773829589No Hit
GTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCACTTTCTAAAGT17550.12279055709131209No Hit
GTAATAAGTTGCAGAATCGTCAGGCTGCAGACTACTGATGGTGAGAGTGGATTCTGTCCCAGATCCACTGCCG17450.1220908957973445No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATCCACTCTCACCATCAGTAGT17360.12146120063277369No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17100.11964208126845795No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17010.1190123861038871No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16960.11866255545690332No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16930.11845265706871304No Hit
GTAATAAATAGCCGTGTCTGCGGCGGTCACAGAGCCCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCAGCGGA16720.1169833683513811No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16530.11565401189284268No Hit
ACACTATGGTGTTTATTCCCGAGTATTCACTTTCGGCCCCGGGACCAGAT16400.1147444522106848No Hit
CATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATCCACTCTCACCATCAGTAGTCTGCAGCCTGACGATTCTG15960.1116659425172274No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15920.11138607799964037No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAATCTGGTCC15670.1096369247647214No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15360.10746797475342186No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15310.10711814410643808No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15190.10627855055367696No Hit
CACTCGGACTGGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGATGCA15120.10578878764789966No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15100.10564885538910612No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15070.10543895700091585No Hit
GTGTTACTGGTTCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCTTGATCTATAAGTCGTCGA15050.10529902474212234No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14830.10375976989539364No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14800.10354987150720336No Hit
ATCTATAAGTCGTCGACTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATCCACT14800.10354987150720336No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14720.1029901424720293No Hit
ATCTGGGACAGAATCCACTCTCACCATCAGTAGTCTGCAGCCTGACGATT14410.10082119246072976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAA200.00654324753.3112358
GTGGTAT3850.042.2994041
AAAACCG1252.9930925E-740.10268870
GTATCAA24750.032.7624551
GACAGGT2450.031.8504451
ATCAACG27800.028.56283
TCAACGC28000.028.3607674
GTGTATG605.9804146E-428.2730391
TTAGTCG500.00706401628.11826152
TATCAAC28500.028.0974222
AACGCAG28400.028.0808126
CAACGCA28450.028.0314625
TGTTAGA18700.027.57251470
ACGCAGA29650.026.8969677
CGCAGAG29550.026.8731468
AGAGTAC30150.026.56068211
CAGAGTA31100.025.75114610
GCAGAGT31300.025.5874969
GCTTGTA801.0714943E-425.4457341
ACGGGTC1101.3319896E-624.678973
Produced by FastQC (version 0.11.5)