FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533108
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA48230.3145897092703188No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39650.2586249631467581No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37880.24707978824714238No Hit
GCCCCAGAGCTGGTGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT37680.24577524871046266No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAC37380.24381843940544304No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT35950.23449098171818292No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31930.2082697370309202No Hit
GCTCTGGGGCTGAGCGCTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACTGTCAGAC30890.20148613144018554No Hit
GAAATATACGGCCGTGTCCGCGGCTCGCAGGCTGTTCATTAGCAGATACA30400.1982900095753202No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29240.19072368026257772No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28880.1883755090965542No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28570.1863534728147006No Hit
TTGTTAACCCTCATCAAGAACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGGTGCTGTGC28490.1858316570000287No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28300.18459234444018296No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28210.18400530164867707No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC27510.17943941327029797No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA26830.17500397884558686No Hit
CAGTAATAGTCACCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCCAGAGCTGGTGC26760.17454739000774896No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25400.16567652115832676No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC24890.1623499453397934No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24810.16182812952512152No Hit
CCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATCAG24700.16111063277994767No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24680.16098017882627969No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT24130.1573926951004104No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23280.15184840206952152No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23030.15021772764867183No Hit
GGGTTAACAATGATGGCAGTCACAGCAAGGGGGACGGGATCCCTGATCGC22960.14976113881083394No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22930.149565457880332No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22690.14800001043631628No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATC22670.14786955648264832No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22010.1435645760116052No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21970.14330366810426926No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21950.14317321415060127No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCACCAGCTCTGGGGCTGAGCGCTCCCTCACCATCTCCAG21750.14186867461392152No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21360.13932482251739603No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT21360.13932482251739603No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA21310.1389986876332261No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21270.13873777972589013No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG21150.1379550560038823No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21040.13723755925870845No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20870.1361287006525307No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20770.1354764308841908No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20480.1335848485560052No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19890.12973645692279998No Hit
CTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACT19460.12693169691893852No Hit
GTAATAGTCACCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGC19340.12614897319693066No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19280.12575761133592675No Hit
CCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCACCCTCATCCTCA19250.1255619304054248No Hit
GTTCTTGATGAGGGTTAACAATGATGGCAGTCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCACCAG19120.12471397970658298No Hit
GTATATTTCTGTGCGAAAGCCCCCGCTCGAGGTGGCGTCGCTGACTACTGGGGCCAGGGAACCCTGGTCACCGT18970.12373557505407316No Hit
GCCCTGGGACTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA18520.12080036109654375No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC18290.11930014062936205No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18180.1185826438841882No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18000.11740855830117644No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17950.1170824234170065No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17850.11643015364866663No Hit
GGACTGGAGTGGGTCTCAGCTCTTAGTGGTAGTGGTGGTAGCACATACTA17550.11447334434364703No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGCTGC17470.11395152852897512No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17460.11388630155214113No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCCAGAGCTGGTGCCTGAGAAGCGATCAGGGATC17000.11088586061777773No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCACC16990.11082063364094377No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT16960.1106249527104418No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16830.10977700201159997No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC16690.10886382433592415No Hit
GGGTTCCCTGGCCCCAGTAGTCAGCGACGCCACCTCGAGCGGGGGCTTTCGCACAGAAATATACGGCCGTGTCCG16690.10886382433592415No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16640.10853768945175421No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG16620.10840723549808623No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16520.10775496572974638No Hit
AGCAGATACAGCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATGTG16380.10684178805407056No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16380.10684178805407056No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16290.10625474526256466No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT16270.10612429130889671No Hit
CCTTGGGGCTGGTCGGGGATGCCGAAGAGACGGTGACCAGGGTTCCCTGG16260.10605906433206272No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT16260.10605906433206272No Hit
GTATCTGCTAATGAACAGCCTGCGAGCCGCGGACACGGCCGTATATTTCTGTGCGAAAGCCCCCGCTCGAGGTGG16260.10605906433206272No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC16220.10579815642472676No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16190.1056024754942248No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16050.104689297818549No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15990.10429793595754507No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15890.1036456661892052No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15720.10253680758302741No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAC15560.10149317595368364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)