FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533108
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC42450.2768885166602744No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA41340.2696483222317019No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC41030.2676262859498483No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40240.2624733547799633No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAC33280.21707537890350842No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT32570.21244426354829535No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29630.1932675323591032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29530.19261526259076334No Hit
GCCCCAGAGCTGGTGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT28330.1847880253706849No Hit
GAAATATACGGCCGTGTCCGCGGCTCGCAGGCTGTTCATTAGCAGATACA28130.18348348583400517No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA27620.18015691001547182No Hit
GCTCTGGGGCTGAGCGCTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACTGTCAGAC27140.17702601512744048No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26690.17409080116991107No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC26320.17167740302705353No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25930.16913355093052804No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25660.16737242255601043No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG25570.16678537976450453No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24860.16215426440929145No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24370.15895814254442608No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24300.1585015537065882No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT24050.1568708792857385No Hit
TTGTTAACCCTCATCAAGAACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGGTGCTGTGC23960.15628383649423264No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT23780.15510975091122087No Hit
GGGTTAACAATGATGGCAGTCACAGCAAGGGGGACGGGATCCCTGATCGC23770.1550445239343869No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23180.15119613230118165No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23140.15093522439384569No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22780.14858705322782217No Hit
CAGTAATAGTCACCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCCAGAGCTGGTGC22540.1470216057838065No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22370.1459127471776287No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC22190.14473866159461696No Hit
CCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATCAG21960.14323844112743525No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21940.14310798717376727No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21830.14239049042859342No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA21440.13984663833206792No Hit
GTTCTTGATGAGGGTTAACAATGATGGCAGTCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCACCAG21250.13860732577222218No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATC21070.1374332401892104No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20710.1350850690231869No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC20610.13443279925484702No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20430.13325871367183525No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20270.13221508204249147No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20230.1319541741351555No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT19570.1276491936641124No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCACCAGCTCTGGGGCTGAGCGCTCCCTCACCATCTCCAG19500.12719260482627448No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19390.1264751080811006No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19380.12640988110426662No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19020.1240617099382431No Hit
GTATATTTCTGTGCGAAAGCCCCCGCTCGAGGTGGCGTCGCTGACTACTGGGGCCAGGGAACCCTGGTCACCGT19020.1240617099382431No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18910.12334421319306925No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18900.12327898621623526No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18580.12119172295754768No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18500.12066990714287577No Hit
GCCCTGGGACTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA18420.12014809132820388No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18190.11864787086102219No Hit
GGGTTCCCTGGCCCCAGTAGTCAGCGACGCCACCTCGAGCGGGGGCTTTCGCACAGAAATATACGGCCGTGTCCG18150.11838696295368625No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG18070.11786514713901435No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17430.11369062062163919No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17240.11245130806179343No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC17050.11121199550194769No Hit
GTATCTGCTAATGAACAGCCTGCGAGCCGCGGACACGGCCGTATATTTCTGTGCGAAAGCCCCCGCTCGAGGTGG16800.109581321081098No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGCTGC16580.1081463275907503No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16520.10775496572974638No Hit
GGGTTAACAATGATGGCAGTCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCACCAGCTCTGGGG16250.10599383735522873No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16220.10579815642472676No Hit
CTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACT15760.10279771549036337No Hit
GTCTGAGGATGAGGGTGACTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAG15640.10201499176835552No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT15590.10168885688418558No Hit
CCTTGGGGCTGGTCGGGGATGCCGAAGAGACGGTGACCAGGGTTCCCTGG15550.10142794897684965No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC15510.10116704106951369No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15510.10116704106951369No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCCAGAGCTGGTGCCTGAGAAGCGATCAGGGATC15500.10110181409267972No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15430.1006452252548418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA200.00705789852.30051450
GGTATCA11650.040.9242551
GTGGTAT3500.039.892791
GTATCAA28650.038.5122641
CACTAGC400.002754958334.0859624
TGGTATC4100.034.0548252
TATCAAC32050.034.0016982
ATCAACG32900.033.3324283
TCAACGC33100.032.9306144
CAACGCA33650.032.3913155
AACGCAG34100.032.063756
CTCGTTA802.329949E-630.85388257
GTAGCGT450.004600410330.69759242
CGCAGAG36550.029.9144738
AGAGTAC37500.029.4233711
GTAGTAG1050.006135514528.93941970
CAGAGTA37900.028.84797510
GCAGAGT38000.028.7729999
CTCGCTA605.8166194E-428.40775123
TCGCTAT756.6930756E-527.2687724
Produced by FastQC (version 0.11.5)