FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575193
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGTCATTCGACGTTTGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCTT95500.6062749136137604No Hit
CTCTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGCTGATGGTGAGAGTGAATT93160.5914195911231195No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58870.37373198077949815No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54100.34344997724088416No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38150.24219254402476398No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38040.24149421689913553No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37830.24016104693202672No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCACAGCTCCTGGGGCTC35060.2225759002230203No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34080.21635444037651258No Hit
CAGTATAAGAGTCATTCGACGTTTGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT32810.20829193628971182No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACTCAGTCTCCTTCCACC32040.20340364641031292No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31530.20016594791876294No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31510.2000389793504669No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31180.19794399797358164No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30210.19178602241122197No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30050.1907702738648534No Hit
GAGCATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAATTACTGATCTATCAGGCGT29690.18848483963552404No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29280.1858819839854545No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28430.18048581983287126No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG28390.18023188269627913No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGCATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA28040.1780099327510978No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27790.17642282564739686No Hit
CTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGC27500.17458178140710376No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCCGATGATTTTGCAACTTA26490.16816986870815195No Hit
GCATGGACATGAGGGTCCCCGCACAGCTCCTGGGGCTCCTGCTGCTCTGG24760.15718708755054142No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24750.15712360326639338No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24460.1552825590261003No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT23440.14880716204300043No Hit
CAGCATGGACATGAGGGTCCCCGCACAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT23010.1460773378246348No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22910.14544249498315445No Hit
GGACACAGCATGGACATGAGGGTCCCCGCACAGCTCCTGGGGCTCCTGCT22780.14461719928922998No Hit
ACTTATTACTGCCAACAGTATAAGAGTCATTCGACGTTTGGCCAAGGGAC21110.13401532383650766No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21050.13363441813161941No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20980.13319002814258316No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20900.13268215386939886No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20830.1322377638803626No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20760.13179337389132634No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20590.1307141410608097No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20260.12861915968392443No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCACAGCTCCTGG20160.12798431684244407No Hit
GGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18910.12004878132393934No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTGATAGATCAGTAATTTAGGGGCTTTCCCTGGTTTCTG18800.11935045419831095No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18140.11516049144454045No Hit
GAGTGAATTCTGTCTCAGATCCACCGCCGCTGAACCTTGATGGGACCCCA18050.11458913288720811No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAATTACTGATCTATCAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT17980.11414474289817185No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17650.1120497615212866No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17580.11160537153225034No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17530.11128795011151014No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17470.11090704440662191No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17400.11046265441758565No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17310.10989129586025331No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17040.10817722018825629No Hit
CATCAAGGTTCAGCGGCGGTGGATCTGAGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCCGATGATTTTG17030.10811373590410825No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16470.10455861599181814No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCACAGCTCCTGGGGCTCC16320.10360635172959759No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT16060.10195576034174861No Hit
GTCCCCGCACAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACTCAGTCT16050.10189227605760057No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16030.10176530748930447No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15910.10100349607952804No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15790.10024168466975159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT601.1014163E-769.2655170
AAGTCGT200.007322341751.8163142
CATACGT200.007676155351.2015433
TCGTATT352.296556E-549.20078340
CGCGTAT800.047.28709439
TCGCGTA851.8189894E-1244.43106538
TATACAC6950.040.4645583
ATCGCGT955.456968E-1239.6928237
CGTATGC4050.039.1783441
AATCGCG1050.039.12581336
TATGGCG801.5970727E-938.922743
TCGTATG3750.037.65540
CGCTCAT7000.037.35546528
ACGCTCA7000.037.3317127
CACGAGA7200.037.192521
GAGACGC7150.036.99025324
ACGAGAC7150.036.9796822
GACGCTC7150.036.5369126
AGACGCT7450.035.96842225
CCCACGA7350.035.96772419
Produced by FastQC (version 0.11.5)