FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575193
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGTCATTCGACGTTTGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCTT93890.5960539438659263No Hit
CTCTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGCTGATGGTGAGAGTGAATT92670.5883088611998657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63060.4003318958375259No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58420.3708751879928364No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45780.29063105282971674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40830.25920633217643807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40220.2553337908434078No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34960.2219410573815399No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACTCAGTCTCCTTCCACC32450.20600650206038246No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCACAGCTCCTGGGGCTC31610.2006738221919473No Hit
CAGTATAAGAGTCATTCGACGTTTGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT31060.1971821865638052No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30980.1966743122906209No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30900.1961664380174366No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30270.19216692811611022No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29820.18931013532944851No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29030.18429487688175353No Hit
GAGCATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAATTACTGATCTATCAGGCGT28920.18359654975612513No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28530.18112066267435165No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGCATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA27960.1775020584779135No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27700.17585146709006452No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA26440.16785244728741178No Hit
CTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGC25990.16499565450075007No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG25690.16309112597630893No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25320.16074220746283152No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCCGATGATTTTGCAACTTA24660.15655224470906107No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24270.15407635762728758No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23840.15134653340892196No Hit
GGACACAGCATGGACATGAGGGTCCCCGCACAGCTCCTGGGGCTCCTGCT23520.14931503631618476No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23290.14785489778077987No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23100.14664869638196715No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23000.1460138535404868No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22760.14449023072093387No Hit
GCATGGACATGAGGGTCCCCGCACAGCTCCTGGGGCTCCTGCTGCTCTGG21840.1386496765793144No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT21580.13699908519146542No Hit
CAGCATGGACATGAGGGTCCCCGCACAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21330.13541197808776448No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21160.13433274525724784No Hit
GAGTGAATTCTGTCTCAGATCCACCGCCGCTGAACCTTGATGGGACCCCA20260.12861915968392443No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20200.12823825397903624No Hit
ACTTATTACTGCCAACAGTATAAGAGTCATTCGACGTTTGGCCAAGGGAC20000.12696856829607545No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19870.12614327260215097No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18930.12017574989223542No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18920.12011226560808738No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCACAGCTCCTGG18770.11916000134586684No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAATTACTGATCTATCAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT18430.11700153568483353No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18300.11617623999090904No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18170.11535094429698456No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17880.11350990005669147No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17320.10995478014440135No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTGATAGATCAGTAATTTAGGGGCTTTCCCTGGTTTCTG17270.10963735872366116No Hit
GGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATC17200.1091929687346249No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16820.10678056593699946No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16500.10474906884426227No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16490.10468558456011423No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16340.10373332029789364No Hit
GTCCCCGCACAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACTCAGTCT16240.10309847745641329No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15790.10024168466975159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4350.038.074871
GTATCAA36750.033.2033121
GACAGGT3750.030.6463851
ATCAACG41500.029.076223
TCAACGC41550.029.0430744
AACGCAG42750.028.6222696
CAACGCA42450.028.506045
TATCAAC42450.028.5051362
TCTTCGG3650.027.77673370
ACGCAGA44550.027.5416857
CGCAGAG44750.027.4941278
AGAGTAC46300.026.6407811
CAGAGTA46700.026.3452510
GCAGAGT46900.026.2337349
AATGGTA5350.025.71851270
AGGTATC2150.025.1535512
AAGGGAC35500.025.02089927
GACGTTT36700.024.5168416
CAAGGGA36150.024.4624226
GATTCAC3600.024.4119511
Produced by FastQC (version 0.11.5)