FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670476
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT87600.5244014280959439No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC75250.4504704048426915No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT67360.40323835840802263No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAGTTGTACAAAACATT57840.34624861416745883No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56460.33798749577964604No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53320.31919045828853576No Hit
CTCCTAAGTTGCTCATTCACTGGGCATCTACCCGGAAATCCGGGGTCCCT52890.31661634168943464No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51890.3106300240171065No Hit
TTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTT50600.3029076742198032No Hit
ATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA49790.29805875690521744No Hit
ATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA46200.27656787646155945No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46200.27656787646155945No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45950.27507129704347744No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40880.2447206664447738No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40400.24184723396205632No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTGTA38820.23238885203977788No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTCACTGGGCATCTACCCGGAAA38610.231131725328589No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT38320.2293956932036138No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38290.22921610367344397No Hit
CACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCCGTGGACGTT38020.2275997979019154No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36900.22089512210890788No Hit
GTTTTGTACAACTCCAACAATAAAAACTACTTAGCTTGGTACCAGCAGAA36890.22083525893218459No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36640.21933867951410257No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA36170.21652511020810836No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35650.21341222501849774No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTGTACAACTCCAA35610.2131727723116046No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35400.21191564560041568No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33800.2023375373246907No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC33360.19970355754886632No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC32650.19545327200151336No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31840.19060435468692757No Hit
ATATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT30460.18234323629911475No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC30320.1815051518249888No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC29680.1776739085146988No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28780.1722862226096035No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28330.16959237965705584No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27920.16713798941140132No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27610.16528223093297958No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27370.16384551469162084No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27300.16342647245455785No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAGTTG27130.1624087984502621No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27090.16216934574336894No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTGTACAACTCCAACAATAAAAACTA26980.16151085079941285No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC26860.1607924926787335No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA26270.1572605652520599No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACT25980.15552453312708472No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25950.1553449435969149No Hit
GTCAGGGACCCCGGATTTCCGGGTAGATGCCCAGTGAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA25630.1534293219417699No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23920.1431927187220888No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCCGT23850.1427736764850258No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT23310.13954106494196863No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22550.13499146351099925No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22100.1322976205584516No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21870.13092076749381612No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGTATT21810.13056158843347646No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21800.13050172525675316No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21440.12834665089471503No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG21320.12762829277403567No Hit
TCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATAT21310.12756842959731238No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21290.1274487032438658No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG21130.12649089241629333No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20910.12517390252838112No Hit
CCCTTACACTCTCCTGTGCAGCCTCTGGCTTCACTTTCAGTGACGCCTAT20760.12427595487753192No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20720.12403650217063877No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20330.12170183827843083No Hit
TCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC20240.1211630696879213No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19940.11936717438622284No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19890.11906785850260644No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT19830.11870867944226676No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19370.11595497331299581No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19310.11559579425265613No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19310.11559579425265613No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19270.11535634154576302No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18900.1131414040070016No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18750.11224345635615236No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18700.11194414047253597No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18530.1109264664682402No Hit
GTACAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18060.10811289716224598No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17870.10697549680450363No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17780.10643672821399411No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17420.10428165385195597No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17410.10422179067523268No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17340.10380274843816972No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17330.10374288526144644No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17210.10302452714076706No Hit
CTCCTGTGCAGCCTCTGGCTTCACTTTCAGTGACGCCTATATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG17200.10296466396404377No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAGGATG17120.10248575855025753No Hit
CAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17100.10236603219681098No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC17050.10206671631319457No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16860.10092931595545222No Hit
ACCATCAACTGCAAGTCCAGCCAGAATGTTTTGTACAACTCCAACAATAA16800.10057013689511254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA1400.044.5132843
GGTATCA11900.042.6018371
AAAACCG1003.6480724E-641.71222370
TAACGGC1400.041.6532236
CGGTACC1600.041.21216245
GTGGTAT3000.039.6951
TCGTATG4700.039.61607440
ACGAGAC5050.039.07739622
CGTATGC4950.039.06888241
CACGAGA5200.038.60100621
ATGCCGT4850.037.8741344
CCCACGA5400.037.79570819
TTAACGG1650.037.3883435
GTATCAA30900.037.2175061
ATCTCGT4300.036.72579237
GCGGTAC1700.036.69713244
GACTAGG5300.036.61956826
TAGGCAT5600.035.9309429
GTATGCC5300.035.87216642
TCTCCGA5700.035.8021711
Produced by FastQC (version 0.11.5)