FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670476
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT88140.5276340396390011No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT78980.4727993697604755No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60600.3627708509430845No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56570.33864599072360213No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAGTTGTACAAAACATT54320.32517677596086386No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53670.32128566947385057No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51490.30823549694817526No Hit
ATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA49710.2975798514914312No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47600.28494872120281883No Hit
TTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTT47350.2834521417847368No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46960.28111747789252883No Hit
CTCCTAAGTTGCTCATTCACTGGGCATCTACCCGGAAATCCGGGGTCCCT46450.2780644558796415No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT44670.26740881042289744No Hit
ATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA43800.26220071404797196No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40320.24136832854827006No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38180.22855760872948788No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38100.22807870331570165No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA36790.2202366271649518No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA36110.21616593114776866No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34720.20784494958323257No Hit
CACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCCGTGGACGTT34650.2074259073461696No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34310.20539055933757802No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTGTA34190.20467220121689866No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34080.20401370627294255No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33850.2026368532083071No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33320.1994641048419732No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32720.19587231423857632No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC32310.1934179239929218No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTCACTGGGCATCTACCCGGAAA32250.1930587449325821No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31610.1892275016222921No Hit
GTTTTGTACAACTCCAACAATAAAAACTACTTAGCTTGGTACCAGCAGAA31360.18773092220421006No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30930.18515680560510897No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC30900.1849772160749391No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC30630.18336091030341053No Hit
ATATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT30560.18294186806634757No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30190.18072693052758615No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTGTACAACTCCAA29870.17881130887244115No Hit
TCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC29550.17689568721729615No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27510.16468359916574676No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27470.16444414645885364No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTTCCGGGT27290.16336660927783458No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26580.1591163237304816No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCCGT26340.15767960748912285No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAGTTG26220.1569612493684435No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGTATT25880.15492590135985193No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25870.15486603818312863No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACT25850.15474631182968207No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25450.15235178476075084No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24860.14881985733407724No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC24680.1477423201530582No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG24400.14606615120480632No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC23910.14313285554536553No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23900.1430729923686422No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTGTACAACTCCAACAATAAAAACTA23710.1419355920108999No Hit
GTCAGGGACCCCGGATTTCCGGGTAGATGCCCAGTGAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA23060.1380444855238866No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23010.1377451696402702No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22790.13642817975235802No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22550.13499146351099925No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22510.13475201080410615No Hit
CAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22200.13289625232568442No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20780.12439568123097848No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20430.12230047004566362No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20410.12218074369221707No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20110.12038484839051863No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19920.11924744803277629No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG19680.11781073179141753No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19660.11769100543797097No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19540.11697264731729161No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19460.11649374190350534No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG19220.1150570256621466No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19210.11499716248542331No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC19060.11409921483457411No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19020.11385976212768098No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG18980.11362030942078784No Hit
CCCTTACACTCTCCTGTGCAGCCTCTGGCTTCACTTTCAGTGACGCCTAT18730.11212373000270581No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18670.11176455094236612No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18380.11002851881739097No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18110.10841221304586238No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18100.1083523498691391No Hit
TCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATAT17840.10679590727433379No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTC17750.10625713868382425No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17730.10613741233037768No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17410.10422179067523268No Hit
CTCCTGTGCAGCCTCTGGCTTCACTTTCAGTGACGCCTATATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG17400.1041619274985094No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17180.10284493761059722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT406.941361E-571.90198570
GGTATCA14250.045.2429921
GTGGTAT4050.044.4051551
TGGTATC4750.039.291142
GTATCAA33100.035.2649271
AATGGTA4400.032.6827270
CCTATAC751.9011932E-631.6691463
ATCAACG39050.030.2383273
GCGTAAG450.005014135530.1619931
TCAACGC39800.029.6729534
AACGCAG40850.029.2442996
TATCAAC40200.029.1218262
CAACGCA40450.029.1139775
GACAGGT3450.028.5227551
TTCACGG5300.028.48946670
CGCAGAG42450.028.3019378
ACGCAGA42900.027.8468447
AGAGTAC42950.027.56993911
GCAGAGT44050.027.2739459
CAGAGTA43850.027.16533910
Produced by FastQC (version 0.11.5)