FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974011
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26890.2760749108582963No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26600.27309753175272145No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCATGTTGTTCATTTGTAGATACA20860.21416595911134476No Hit
GTACAATTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT19580.20102442374880777No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18300.1878828883862708No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCATGTTGTTCATTTGTAGATA17720.18192813017512122No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGTTGATTGTCAGTGTGAAATCTGTCCTCGCCCCACTGCCA16360.1679652488524257No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16220.16652789342214822No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16190.16621988868708873No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16160.1659118839520293No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14990.15389969928471034No Hit
GTTTATTACTGCATGCATGCTGTACAATTGGGGACGTTCGGCCAAGGGAC14860.15256501209945267No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT14430.1481502775636004No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14300.14681559037834274No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14020.14394087951778778No Hit
CTATGTGGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACGCCAAGGACTCGCTGTATCTACAAATGAA13960.14332487004766886No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13880.14250352408751032No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13670.1403474909420941No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT13160.13511141044608327No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12940.13285270905564722No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12730.13069667591023099No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12610.12946465696999315No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12490.12823263802975532No Hit
GTGTATTACTGTGTGAGAGATGAGGGTGGACTGCCTTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC12420.12751396031461656No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12270.12597393663931927No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12100.12422857647398232No Hit
CCCTTGGGCAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTT11900.12217521157358592No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA11750.12063518789828863No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11670.11981384193813006No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11510.11817115001781293No Hit
ATTGTACAGCATGCATGCAGTAATAAACCCCGACATCCTCAGCTTCCACCCTGTTGATTGTCAGTGTGAAAT11500.11806848177279311No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11460.11765780879271384No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10980.11272973303176248No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10880.11170305058156428No Hit
ATATAAAGCCAGATGGAAGTGAGACATACTATGTGGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACG10860.11149771409152463No Hit
GACAATCAACAGGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCATGCTGTACAATTGGGGACGTTCGG10730.11016302690626696No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10650.10934168094610841No Hit
CTGTACAATTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA10560.10841766674093002No Hit
AATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTC10520.10800699376085074No Hit
CTCCTCACCTGTCACCCTTGGGCAGCCGGCCTCCATCTCCTGCAGGTCTA10310.10585096061543453No Hit
GTCTGGGACCCCAGAGAACCGGGTGGAAACCTCATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGTTGAAG10220.10492694641025616No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC10160.10431093694013722No Hit
CAATTGTACAGCATGCATGCAGTAATAAACCCCGACATCCTCAGCTTCCACCCTGTTGATTGTCAGTGTGAAAT10040.10307891799989938No Hit
GTATTACTGTGTGAGAGATGAGGGTGGACTGCCTTTTGACTACTGGGGCC9960.10225757203974081No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGCGTCCAGTGTGAATTGCAGCTGGTGGAGT9920.10184689905966154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT800.047.3522339
TATACAC4800.046.8223153
CTTATAC4800.046.1294631
GGTATCA8950.045.673521
CTCGACG4650.044.79070328
GACGTCA4700.044.39647731
TCGACGT4700.044.33703229
CACGAGA5050.043.1690621
CGACGTC4850.043.00121730
CGAGACT5150.043.00107623
GACTCGA5000.042.97244626
TCGTATG3050.042.9598740
ACGAGAC5250.042.17334422
AGACTCG5200.041.29205725
TTATACA5350.040.7358132
CGTATGC3500.040.4382141
CCGTCTT3100.040.41422347
ATCTCCG5400.040.3586310
ACGTCAT4250.040.28521732
GAGACTC5450.040.01301224
Produced by FastQC (version 0.11.5)