FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974011
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48120.49403959503537437No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26510.2721735175475431No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19250.19763637166315373No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCATGTTGTTCATTTGTAGATACA18940.19445365606753928No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18060.1854188505057951No Hit
GTACAATTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT17540.18008010176476447No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16520.1696079407727428No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCATGTTGTTCATTTGTAGATA14880.15277034858949232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14460.14845828229865987No Hit
GTTTATTACTGCATGCATGCTGTACAATTGGGGACGTTCGGCCAAGGGAC14440.14825294580862022No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT13730.140963500412213No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGTTGATTGTCAGTGTGAAATCTGTCCTCGCCCCACTGCCA13510.13870479902177696No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13490.13849946253173734No Hit
GTGTATTACTGTGTGAGAGATGAGGGTGGACTGCCTTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC13450.13808878955165804No Hit
CCCTTGGGCAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTT13160.13511141044608327No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13090.13439273273094451No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13080.13429006448592468No Hit
CTATGTGGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACGCCAAGGACTCGCTGTATCTACAAATGAA12960.13305804554568687No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12860.13203136309548866No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA12860.13203136309548866No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11780.12094319263334807No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11430.11734980405765438No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11210.11509110266721835No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11110.11406442021702012No Hit
CTCCTCACCTGTCACCCTTGGGCAGCCGGCCTCCATCTCCTGCAGGTCTA10780.11067636813136607No Hit
ATATAAAGCCAGATGGAAGTGAGACATACTATGTGGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACG10570.10852033498594985No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10530.10810966200587056No Hit
AATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTC10440.1071856478006922No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10340.106158965350494No Hit
ATTGTACAGCATGCATGCAGTAATAAACCCCGACATCCTCAGCTTCCACCCTGTTGATTGTCAGTGTGAAAT10220.10492694641025616No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10050.10318158624491922No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9850.10112822134452282No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC9790.10051221187440389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)