FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631623
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTAGTCCGCGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT40180.6361389626406891No Hit
GTATAATATTGTTGACAGTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC29780.4714837806729647No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25280.4002387500138532No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24610.3896311565601633No Hit
CTCCTAAGCTGCTCATTTCCTGGGCAACTACCCGGGAATCCGGGGTCCCT20170.31933605964317324No Hit
GTTTATTACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAG18720.296379327541904No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18080.2862467009592748No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17850.2826052882811424No Hit
GACCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCAACTACCCGGGAA17210.2724726616985132No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16250.25727372182456937No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT16120.2552155320499728No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16100.25489888746926564No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15620.2472994175322938No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15390.24365800485416142No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14210.22497597459243882No Hit
GCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTAATAAACACT13870.21959301672041706No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13500.21373509197733456No Hit
GTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCGAAGCCA13110.2075605226535449No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12770.20217756478152316No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12630.199961052716573No Hit
CTTTATTACTGTCAACAATATTATACTAGTCCGCGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAA11950.18919513697252952No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11480.1817539893259112No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11420.1808040555837897No Hit
ATACTAGTCCGCGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGA10870.17209632961434274No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10300.1630719590641886No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCTCTTTATTACTGTCAACAATATTATACTAGTCCGCGGACTTT9890.15658074515969178No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG9600.15198939873943793No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9330.14771469689989122No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9280.14692308544812333No Hit
GTAATAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCCCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA9010.14264838360857662No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8970.14201509444716232No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8830.13979858238221216No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8720.13805703718832277No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCTCTTTATTACTGTCAACAATATTATACTAGT8650.13694878115584772No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8640.13679045886549412No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCAACTACCCGGGAATCCGGGGTCCCTGA8320.13172414557417952No Hit
GACTAGTATAATATTGTTGACAGTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT8190.12966595579958298No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8140.12887434434781506No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7900.12507460937932913No Hit
GAGTGAAATCTGTCCCAGATCCGAAGCCACTGAATCGGTCAGGGACCCCG7880.12475796479862196No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7830.12396635334685406No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7720.12222480815296467No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7610.12048326295907527No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTC7590.1201666183783681No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7560.11969165150730737No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7500.11874171776518588No Hit
GTCCCTGACCGATTCAGTGGCTTCGGATCTGGGACAGATTTCACTCTCAC7480.11842507318447872No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7450.11795010631341796No Hit
ATTCAGTGGCTTCGGATCTGGGACAGATTTCACTCTCACCATCACCAGCC7440.11779178402306438No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7430.1176334617327108No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGGGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTATTACAGCTCCAA7380.11684185028094292No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7360.11652520570023574No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7350.11636688340988216No Hit
GCTCATTTCCTGGGCAACTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCTTCGGATCTGGGACAGATTT7350.11636688340988216No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7290.11541694966776068No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCGAAGCCACTGAATCGGTCAGGGACC7280.11525862737740708No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7240.11462533821599277No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7210.11415037134493203No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7060.1117755369896283No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7000.11082560324750682No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6990.11066728095715324No Hit
CCCTGGCTGTGTCTCTGGGCGAGGGGGCCACCATCAACTGCAAGTCCAGC6890.10908405805361743No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6850.10845076889220309No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6820.10797580202114236No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6810.10781747973078878No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC6360.10069297666487763No Hit
CTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCA6350.10053465437452405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCA200.00717890652.0724944
GTGGTAT1800.043.3355331
TCGTATG3850.042.98823540
GGTATCA8450.042.9453281
GATCTCG3300.042.60223436
TCGGTAG405.445698E-542.50456628
GTATCAA25100.042.2921141
CGTATGC4000.041.4529641
ATCTCGT3400.041.4022837
GCCGTCT4000.040.90620446
ATGCCGT4000.040.79011544
CCGTCTT4000.040.11087447
ACATGCG3900.040.0877727
TATACAC4200.039.5421683
AGATCTC3550.039.52926335
ATGCGCA4000.039.12906329
TCTCCGA4000.038.9772811
CTTATAC4200.038.759731
CATGCGC4050.038.62143328
CGTCTTC4200.038.27879348
Produced by FastQC (version 0.11.5)