FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631623
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTAGTCCGCGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT40630.6432634657066003No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34090.5397206878153582No Hit
GTATAATATTGTTGACAGTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC28550.45201013895947423No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25980.41132131033860386No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20410.32313579461165914No Hit
CTCCTAAGCTGCTCATTTCCTGGGCAACTACCCGGGAATCCGGGGTCCCT19780.31316149031938356No Hit
GTTTATTACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAG19740.3125282011579692No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19370.3066702764148867No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17080.27041447192391665No Hit
GACCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCAACTACCCGGGAA16150.2556904989210336No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15400.24381632714451498No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13480.2134184473966274No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13340.21120193533167728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12840.2032858208139982No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12720.20138595332975526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12580.19916944126480512No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12340.1953697062963192No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11960.18935345926288308No Hit
CTTTATTACTGTCAACAATATTATACTAGTCCGCGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAA11930.18887849239182233No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11870.18792855864970087No Hit
GTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCGAAGCCA11720.18555372429439715No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11160.1766876760345966No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10070.15943054638605625No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9920.15705571203075253No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9800.15515584454650955No Hit
ATACTAGTCCGCGGACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGA9660.15293933248155941No Hit
GCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTAATAAACACT9570.15151443186837718No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9420.14913959751307346No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCTCTTTATTACTGTCAACAATATTATACTAGT9290.14708140773847692No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8800.13932361551115144No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCTCTTTATTACTGTCAACAATATTATACTAGTCCGCGGACTTT8700.1377403926076156No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGGGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTATTACAGCTCCAA8490.1344156245101904No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8230.1302992449609973No Hit
GACTAGTATAATATTGTTGACAGTAATAAAGAGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT7940.12570789854074343No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7920.12539125396003628No Hit
GAGTGAAATCTGTCCCAGATCCGAAGCCACTGAATCGGTCAGGGACCCCG7780.12317474189508615No Hit
ATTCAGTGGCTTCGGATCTGGGACAGATTTCACTCTCACCATCACCAGCC7780.12317474189508615No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7750.1226997750240254No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7740.12254145273367183No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7710.12206648586261107No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7560.11969165150730737No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7470.11826675089412514No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCAACTACCCGGGAATCCGGGGTCCCTGA7420.11747513944235723No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7410.11731681715200365No Hit
GTCCCTGACCGATTCAGTGGCTTCGGATCTGGGACAGATTTCACTCTCAC7350.11636688340988216No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGGGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTATTA7300.11557527195811426No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7270.1151003050870535No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7240.11462533821599277No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7220.11430869363528562No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7050.11161721469927473No Hit
GCTCATTTCCTGGGCAACTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCTTCGGATCTGGGACAGATTT7030.11130057011856757No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTC6980.11050895866679966No Hit
CCCTGGCTGTGTCTCTGGGCGAGGGGGCCACCATCAACTGCAAGTCCAGC6780.10734251285972804No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6610.10465103392371716No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG6600.10449271163336359No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6570.10401774476230283No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6520.10322613331053493No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6470.10243452185876702No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCGAAGCCACTGAATCGGTCAGGGACC6460.10227619956841344No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6380.10100962124558478No Hit
CTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCA6370.1008512989552312No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTCCGCGGACTAGT6360.10069297666487763No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6360.10069297666487763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG6360.10069297666487763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8950.040.4246941
GTATCAA22800.039.7453161
GTCGGTT500.00214098235.87890669
CTCGTAT400.002163582535.80368860
GCACAAT552.3533333E-433.14641665
TATCAAC27400.032.8207362
ATCAACG28000.031.8784713
AACGCAG28600.031.6775516
TCAACGC28100.031.6396884
CAACGCA28750.031.0419435
GTGGTAT2400.030.9954151
CGCAGAG29700.030.3904868
AGAGTAC29800.030.17267411
GCGATTC450.005006543430.1684527
ACGCAGA30250.029.9496847
GATCTGG11450.029.81597170
GCAGAGT30350.029.6282339
TGTTAGA9850.029.4971770
CAGAGTA30550.029.43426910
TCGGTTT1000.006011957329.0547170
Produced by FastQC (version 0.11.5)