FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089964
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54100.49634666833033014No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50940.4673548851154717No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42030.3856090659875005No Hit
CTTCAGTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGT37240.34166266041814225No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36680.33652487605095216No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36540.33524042995915465No Hit
AACCAGGACAGTCTCCTAACTTGCTCTTTTACTGGGCATCAACCCGGAAA35850.3289099456495811No Hit
CCCTGGCAGTGTCTCTGGGCGAGAACGCCACCATCAACTGCAAGTCCAGT34420.3157902462833635No Hit
CTTTTATTCAGTTCCAACAATAAGAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCT34080.31267087720328374No Hit
GTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTCTTATTGTTGGAACTG33930.31129468496207213No Hit
AGTTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTCTTA32250.2958813318605018No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30980.28422957088490997No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30750.2821194094483855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30210.27716511738002353No Hit
CTCCTAACTTGCTCTTTTACTGGGCATCAACCCGGAAAGCCGGGGTCCCT29490.2705593946222077No Hit
GTCAGGGACCCCGGCTTTCCGGGTTGATGCCCAGTAAAAGAGCAAGTTAGGAGACTGTCCTGGTTTCTGCTGGTA29390.2696419331280666No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28670.26303621037025077No Hit
GTCTCTGGGCGAGAACGCCACCATCAACTGCAAGTCCAGTCACAATCTTTTATTCAGTTCCAACAATAAGAACTA28510.261568271979625No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGAACAGATTTCACTCTCAC28330.25991684129017106No Hit
GTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAACGCCACCATCAACTGCAAGTCCAGTCACAATCTTTTATT28240.25909112594544403No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27790.25496254922180916No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27750.2545955646241527No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27680.25395334157825394No Hit
GTGTTGCAGACCCAGGTCTTCAGTACTCTGTTGCTCTGGATCTCTGGTGC26680.24477872663684308No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26550.24358602669445964No Hit
ATATTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26270.24101713451086457No Hit
GAATAAAAGATTGTGACTGGACTTGCAGTTGATGGTGGCGTTCTCGCCCAGAGACACTGCCAGGGAGTCTGGAGA25820.23688855778722967No Hit
GTCTCCTAACTTGCTCTTTTACTGGGCATCAACCCGGAAAGCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGT25770.23642982704015914No Hit
CTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC24330.22321838152452741No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24050.22064948934093237No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23260.21340154353721774No Hit
GTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGG23070.21165836669834967No Hit
ATTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAA23030.21129138210069323No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22990.2109243975030368No Hit
GTAAAAGAGCAAGTTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTA22890.2100069360088957No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCAAAAGTG22680.20808026687119943No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAAC22270.20431867474522092No Hit
ACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCTCTTT22170.20340121325107982No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCAAAAGTGAGAGGAGGATT21650.19863041348154617No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21490.19716247509092044No Hit
TCAGTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTC20870.19147421382724567No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20390.18707039865536843No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20270.18596944486239914No Hit
GGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGT20040.1838592834258746No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAACAATATTATAATCCT19960.18312531423056175No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19850.18211610658700655No Hit
GGATTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT19790.1815656296905219No Hit
CTCCCTGGCAGTGTCTCTGGGCGAGAACGCCACCATCAACTGCAAGTCCAGTCACAATCTTTTATTCAGTTCCAA19650.18028118359872436No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19460.1785380067598563No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19330.17734530681747288No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19290.17697832221981644No Hit
GTTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTCTTAT18730.17184053785262632No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18630.17092307635848525No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18550.17018910716317237No Hit
GGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAACGCCA18520.16991386871493003No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18490.1696386302666877No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18140.16642751503719388No Hit
TTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT18030.1654183073936387No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT17640.16184020756648845No Hit
CTTTTACTGGGCATCAACCCGGAAAGCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGAACAGATTTCA17580.1612897306700038No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17310.15881258463582285No Hit
GTTTATTATTGTCAACAATATTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAA17300.15872083848640872No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCAACAATATTATAATCCTCCTC17130.15716115394636887No Hit
CCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAACAA16800.15413353101570326No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16740.1535830541192186No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCG16690.15312432337214807No Hit
CTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAA16510.1514728926826941No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16250.14908749279792727No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16020.14697733136140279No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15820.1451424083731206No Hit
CTACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCTCTTTTACTGGGCATCAACCCGGAAAGC15770.14468367762605003No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15660.14367446998249483No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15590.14303224693659608No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15530.14248177004011142No Hit
CAATAAGAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCTCTTTTACTGGGCATCAAC15430.14156430854597032No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15140.13890367021296116No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15020.13780271641999187No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14960.1372522395235072No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14950.13716049337409308No Hit
ATTCAGCGGCAGCGGGTCTGGAACAGATTTCACTCTCACCATCAACAGCC14840.1361512857305379No Hit
GTTCCAACAATAAGAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCTCTTTTACTGGG14820.13596779343170967No Hit
CTTAAGTAGTTCTTATTGTTGGAACTGAATAAAAGATTGTGACTGGACTT14720.13505033193756857No Hit
TTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTT14700.13486683963874038No Hit
AGATGGTGTTGCAGACCCAGGTCTTCAGTACTCTGTTGCTCTGGATCTCT14640.13431636274225572No Hit
CAGTAAAAGAGCAAGTTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACT14540.13339890124811463No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14340.13156397825983243No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14100.1293620706738938No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14050.1289033399268233No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13980.1282611168809245No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13980.1282611168809245No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13890.12743540153619753No Hit
CTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA13840.12697667078912697No Hit
CTGCAAGTCCAGTCACAATCTTTTATTCAGTTCCAACAATAAGAACTACT13800.12660968619147053No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCAGTACTCTGTT13800.12660968619147053No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCAGTACTCTGTTGCTCTGGATC13790.12651794004205644No Hit
GTCTGGAACAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATG13700.12569222469732946No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCGCTGAATCGGTCAGGGACC13640.1251417478008448No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13570.12449952475494604No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13470.12358206326080494No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13420.12312333251373439No Hit
CCCAGGTCTTCAGTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGA13360.12257285561724976No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13340.12238936331842154No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13300.1220223787207651No Hit
ACCTTGGTCCCTCCGCCAAAAGTGAGAGGAGGATTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGC13210.1211966633760381No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13140.12055444033013935No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13080.12000396343365469No Hit
CCTTGGTCCCTCCGCCAAAAGTGAGAGGAGGATTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGC12920.11853602504302894No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12890.11826078659478662No Hit
ACCATCAACTGCAAGTCCAGTCACAATCTTTTATTCAGTTCCAACAATAA12840.11780205584771607No Hit
GCAGAAACCAGGACAGTCTCCTAACTTGCTCTTTTACTGGGCATCAACCCGGAAAGCCGGGGTCCCTGACCGATT12830.11771030969830196No Hit
GGTTTCTGCTGGTACCAACTTAAGTAGTTCTTATTGTTGGAACTGAATAA12790.11734332510064552No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12560.11523316366412102No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC12550.11514141751470691No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12430.1140404637217376No Hit
GACCGATTCAGCGGCAGCGGGTCTGGAACAGATTTCACTCTCACCATCAA12340.11321474837701062No Hit
GGTCTTCAGTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTG12240.11229728688286952No Hit
CAATATTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12190.11183855613579897No Hit
CCAGACTCCCTGGCAGTGTCTCTGGGCGAGAACGCCACCATCAACTGCAA12150.11147157153814254No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGC12070.11073760234282969No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11900.10917791780278982No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA11860.10881093320513338No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11840.10862744090630516No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11710.10743474096392175No Hit
GTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCGCTGAATCGGT11700.10734299481450764No Hit
GTGATGACCCAGTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAACGC11180.10257219504497396No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCAAAAGTGAGAGG11110.10192997199907519No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG11010.10101251050493411No Hit
GCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGA10940.10037028745903534No Hit
GTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAG10940.10037028745903534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCT200.006732220852.9279654
GCTTGCG200.00781718750.9652371
GTGGTAT2000.050.9652331
GGTATCA7850.044.148231
TGGTATC2300.042.8226552
GACAGGT1550.041.649011
ATCGCGG350.001380327639.23763737
GTCGTAT451.0115727E-438.26838739
TGTCGTA557.583756E-637.4975838
ATGTCGT557.644359E-637.45410537
GTATCAA20200.036.8362581
CGTATGC2300.036.0530641
TCGTATG2250.035.26268840
CGTAGAG2950.034.6242128
GCTTGAC400.002798261133.976821
CCTAGCT400.002803932433.96279513
ATAAGAC400.002803932433.9627953
GAGACGT3050.033.4505324
TATACAC3100.032.867223
CTTATAC3350.032.455471
Produced by FastQC (version 0.11.5)