FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089964
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55160.5060717601682257No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42420.38918716581465074No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38270.35111251380779546No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38100.3495528292677556No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36130.3314788378331761No Hit
GTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTCTTATTGTTGGAACTG36110.3312953455343479No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32390.2971657779522993No Hit
CTTCAGTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGT31830.2920279935851092No Hit
AGTTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTCTTA31230.2865232246202627No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29470.2703759023233795No Hit
AACCAGGACAGTCTCCTAACTTGCTCTTTTACTGGGCATCAACCCGGAAA28380.2603755720372416No Hit
GTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCGCTGAATCGGT28110.2578984260030606No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCG27970.2566139799112631No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27370.2511092109464166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27360.2510174647970025No Hit
ATATTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT27190.2494577802569626No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCGCTGAATCGGTCAGGGACC26820.24606317272864056No Hit
GTCAGGGACCCCGGCTTTCCGGGTTGATGCCCAGTAAAAGAGCAAGTTAGGAGACTGTCCTGGTTTCTGCTGGTA26750.24542094968274183No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26440.24257681905090445No Hit
CTTTTATTCAGTTCCAACAATAAGAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCT26290.2412006268096928No Hit
CTCCTAACTTGCTCTTTTACTGGGCATCAACCCGGAAAGCCGGGGTCCCT26080.23927395767199652No Hit
CCCTGGCAGTGTCTCTGGGCGAGAACGCCACCATCAACTGCAAGTCCAGT25890.23753078083312845No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCAAAAGTG25550.23441141175304872No Hit
GAATAAAAGATTGTGACTGGACTTGCAGTTGATGGTGGCGTTCTCGCCCAGAGACACTGCCAGGGAGTCTGGAGA25520.23413617330480638No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25490.23386093485656406No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25140.23064981962707026No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCAAAAGTGAGAGGAGGATT24270.22266790462804276No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24210.2221174277315581No Hit
GGATTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT24130.22138345853624525No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGAACAGATTTCACTCTCAC24110.22119996623741703No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24070.2208329816397606No Hit
GTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAACGCCACCATCAACTGCAAGTCCAGTCACAATCTTTTATT23920.21945678939854896No Hit
GAGTGAAATCTGTTCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCG23570.2162456741690551No Hit
GTGTTGCAGACCCAGGTCTTCAGTACTCTGTTGCTCTGGATCTCTGGTGC23290.21367678198546008No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23010.211107889801865No Hit
ATTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAA22540.2067958207794019No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22510.20652058233115955No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21980.20165803641221178No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21810.20009835187217193No Hit
GTAAAAGAGCAAGTTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTA21800.2000066057227578No Hit
GTCTCCTAACTTGCTCTTTTACTGGGCATCAACCCGGAAAGCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGT21790.1999148595733437No Hit
GTTCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCGGCTTTCCGGGT21740.19945612882627314No Hit
GTCTCTGGGCGAGAACGCCACCATCAACTGCAAGTCCAGTCACAATCTTTTATTCAGTTCCAACAATAAGAACTA21600.1981716827344756No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21590.1980799365850615No Hit
CTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC21120.1937678675625984No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20640.18936405239072118No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAACAATATTATAATCCT20120.18459325262118748No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAAC19700.1807399143457949No Hit
GTTTATTATTGTCAACAATATTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAA19570.17954721440341148No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18950.17385895313973673No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18930.1736754608409085No Hit
TTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTT18910.1734919685420803No Hit
TTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT18630.17092307635848525No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18260.1675284688301632No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18170.16670275348543623No Hit
TCAGTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTC18100.16606053043953745No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18090.16596878429012335No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18020.1653265612442246No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17810.16339989210652828No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17760.16294116135945774No Hit
GTTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTCTTAT17650.16193195371590255No Hit
GTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGG17650.16193195371590255No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCAACAATATTATAATCCTCCTC17640.16184020756648845No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17570.16119798452058967No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17500.16055576147469092No Hit
CTCCCTGGCAGTGTCTCTGGGCGAGAACGCCACCATCAACTGCAAGTCCAGTCACAATCTTTTATTCAGTTCCAA17480.1603722691758627No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16970.15569321555574314No Hit
ACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCTCTTT16930.1553262309580867No Hit
GGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGT16540.15174813113093644No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16380.15028019274031068No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15940.1462433621660899No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15830.1452341545225347No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15760.1445919314766359No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15430.14156430854597032No Hit
GGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAACGCCA15430.14156430854597032No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15300.1403716086035869No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15200.13945414710944581No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15160.13908716251178938No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15160.13908716251178938No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14820.13596779343170967No Hit
CCTTGGTCCCTCCGCCAAAAGTGAGAGGAGGATTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGC14800.13578430113288145No Hit
CCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAACAA14690.13477509348932626No Hit
GTCTGGAACAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATG14620.1341328704434275No Hit
ACCTTGGTCCCTCCGCCAAAAGTGAGAGGAGGATTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGC14590.13385763199518516No Hit
CTTTTACTGGGCATCAACCCGGAAAGCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGAACAGATTTCA14250.13073826291510546No Hit
ATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGT14240.13064651676569133No Hit
ATTCAGCGGCAGCGGGTCTGGAACAGATTTCACTCTCACCATCAACAGCC14220.13046302446686311No Hit
CTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA14130.12963730912213614No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCG14120.12954556297272202No Hit
CTTAAGTAGTTCTTATTGTTGGAACTGAATAAAAGATTGTGACTGGACTT14090.1292703245244797No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13590.12468301705377426No Hit
GGTTTCTGCTGGTACCAACTTAAGTAGTTCTTATTGTTGGAACTGAATAA13490.12376555555963316No Hit
GTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAG13440.12330682481256261No Hit
CTACGGGACAATTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAA13390.12284809406549207No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13180.12092142492779578No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13160.12073793262896756No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13140.12055444033013935No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCAGTACTCTGTT13100.1201874557324829No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13050.11972872498541237No Hit
CTGTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCGCTGAATCG13030.11954523268658415No Hit
CAGTAAAAGAGCAAGTTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACT12930.11862777119244305No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12800.11743507125005963No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12770.1171598328018173No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12740.11688459435357497No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12660.11615062515826212No Hit
CCCAGGTCTTCAGTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTGGCTACGGGGACATCGTGATGA12360.11339824067583884No Hit
CAATATTATAATCCTCCTCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12350.11330649452642473No Hit
AGATGGTGTTGCAGACCCAGGTCTTCAGTACTCTGTTGCTCTGGATCTCT12270.11257252533111185No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGC12200.11193030228521308No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCAGTACTCTGTTGCTCTGGATC12160.11156331768755665No Hit
CTACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCTCTTTTACTGGGCATCAACCCGGAAAGC12120.1111963330899002No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12120.1111963330899002No Hit
GTTCCAACAATAAGAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCTCTTTTACTGGG12120.1111963330899002No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12070.11073760234282969No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12010.11018712544634503No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCAAAAGTGAGAGG12010.11018712544634503No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG11980.1099118869981027No Hit
CAATAAGAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAACTTGCTCTTTTACTGGGCATCAAC11940.10954490240044626No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11940.10954490240044626No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11890.10908617165337571No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC11860.10881093320513338No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11810.10835220245806283No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11720.10752648711333586No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11680.10715950251567942No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11550.10596680257329599No Hit
GGTCTTCAGTACTCTGTTGCTCTGGATCTCTGGTGCCTACGGGACAATTG11330.1039483872861856No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11270.10339791038970095No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11120.1020217181484893No Hit
GAATCGGTCAGGGACCCCGGCTTTCCGGGTTGATGCCCAGTAAAAGAGCAAGTTAGGAGACTGTCCTGGTTTCTG11040.10128774895317644No Hit
GATTATAATATTGTTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT11010.10101251050493411No Hit
GACCGATTCAGCGGCAGCGGGTCTGGAACAGATTTCACTCTCACCATCAA10930.10027854130962123No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10910.10009504901079301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTA200.004401351358.9284568
GGTATCA9050.042.6889041
GACAGGA1950.039.9716381
GTGGTAT1950.038.233741
GTATCAA20900.034.861891
ATACTTT502.0646003E-433.896786
GACTGAC552.6658876E-432.45255359
CGTCAAT751.6126178E-632.3422543
ATCAACG23800.030.7606373
CTCGTGT450.00465419730.62392639
TCAACGC24000.030.3658644
GTTGACC450.005041342730.1277033
TATCAAC24200.029.9720862
TGGTATC2500.029.8264242
CAACGCA24600.029.7630215
AACGCAG24750.029.7195996
GACAGGT1600.029.6528741
ACTTTAT3600.029.1888926
ATTCACT3400.028.9093022
CGCAGAG26100.028.1836728
Produced by FastQC (version 0.11.5)