FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176453
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37870.3218998124021954No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAAGTGTAGCTGCCTGCATATGAGCAG35510.3018395125007119No Hit
GTGTCCGGGTCTCCTGGACAATCAGTCACCATCTCCTGCACTGGAACCAG29790.25321878562084504No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACTTTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT28910.2457386737931732No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28400.2414036089839543No Hit
GTAATAGAAGGCGGTGTCCTCAGTTATCAGACTGTTCATTTGCAGATACA27780.23613353019627642No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAAGTGTAGCTGCCTGCATATGAGCAGCAGTAATAAT26680.22678339041168663No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25560.21726324808555886No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23840.20264302951329122No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23540.2000929913902213No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCAC20520.1744226076179839No Hit
GTAATAATCAGCCTCATCCTCAACCTGGAGCCCAGAGATGGTCAGGGAGG20250.17212757330722095No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATCATTTATGATGTCA20150.17127756059953098No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20080.17068255170414798No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19990.169917540267227No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT19840.16864252120569204No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG19090.16226742589801718No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18650.15852736998418127No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCACCATCTCCTG18570.15784735981802928No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18450.1568273445688013No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18270.15529732169495933No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18250.15512731915342134No Hit
GTGTTGGTACCAGGAGACATAGTGATAACCACCAACATCACTGCTGGTTC18150.15427730644573137No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17820.15147226451035445No Hit
GTGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAAC17460.1484122187626705No Hit
CTATGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCATCA17350.14747720478421153No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATCACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGC17350.14747720478421153No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17280.14688219588882853No Hit
TCTCTGGGCTCCAGGTTGAGGATGAGGCTGATTATTACTGCTGCTCATAT17180.14603218318113856No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17170.14594718191036957No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT16620.14127211201807466No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16480.1400820942273087No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16410.13948708533192572No Hit
GTCTGATAACTGAGGACACCGCCTTCTATTACTGTGCAAAAGATGGTGAC16310.13863707262423572No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16170.13744705483346975No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16000.1360020332303968No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15870.13489701671039983No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15790.13421700654424784No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15410.1309869582550259No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15280.12988194173502893No Hit
CTCCTGTGCAGCCTCTGGATTCAACTTTGATGATTATACCATGTACTGGGTCCGTCAAGCTCCGGGGAAGGGT15240.12954193665195296No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15090.12826691759041797No Hit
GTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGACATAGTGATAA15020.12767190869503497No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC14630.12435685913504406No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14400.12240182990735711No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC13920.11832176891044521No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13870.11789676255660021No Hit
CCCCTGAGGGCCGCTTACTGACATCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA13770.11704674984891024No Hit
TTCTATTACTGTGCAAAAGATGGTGACGGTCTGGGGAACACCAACTGGTACTTCGATTTCTGGGGCCGTGGCACC13690.11636673968275825No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13680.11628173841198926No Hit
CAGCTACACTTTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA13670.11619673714122027No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13470.1144967117258403No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCACCATCT13390.11381670155968832No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13170.11194667360277036No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCACCATCTCCTGCA13090.11126666343661837No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCCTGTATCTGCAAATGAA13000.1105016519996974No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12830.10905663039662444No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC12780.10863162404277943No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAAGTGTAG12550.10667659481509248No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGGGTGCCACGG12360.10506157067048152No Hit
CTCCAGACCCTTCCCCGGAGCTTGACGGACCCAGTACATGGTATAATCAT12330.10480656685817452No Hit
GTTATCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCT12250.10412655669202253No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12170.10344654652587056No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCAC12090.10276653635971858No Hit
GGTCTGGAGTGGGTCTCTCTTATTAGTTGGGATGGTGGTAATACATACTA11980.1018315223812596No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11970.10174652111049061No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11970.10174652111049061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4250.072.1334470
GTCGTAT507.038943E-848.21805239
TCTCGTA4200.046.6636838
ATCTCGT4300.046.33233337
TCGTATG5300.045.55144540
CGTATGC5250.045.39679341
TAGCGCT5900.044.51616329
CTAGCGC5900.043.3414328
CGCTCAT5700.043.17988232
CCGTCTT4850.043.08627747
CACGAGA6250.043.0066521
CATCTCG4550.042.97633436
ACGAGAC6100.042.95229322
GTATGCC5650.042.8681942
ACTAGCG6050.042.8030627
GCGCTCA6200.042.41637831
ATGCCGT5350.042.13482744
GACTAGC6250.041.41749626
GCCCACG6450.041.14385618
CCCACGA6450.041.14385619
Produced by FastQC (version 0.11.5)