FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176453
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58990.5014224962663192No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34620.29427439940227107No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAAGTGTAGCTGCCTGCATATGAGCAG34080.2896843307807452No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30330.25780885424237093No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACTTTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT29750.2528787805377691No Hit
GTGTCCGGGTCTCCTGGACAATCAGTCACCATCTCCTGCACTGGAACCAG29670.2521987703716171No Hit
GTAATAGAAGGCGGTGTCCTCAGTTATCAGACTGTTCATTTGCAGATACA25470.21649823664863788No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24460.2079131083009691No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAAGTGTAGCTGCCTGCATATGAGCAGCAGTAATAAT23910.20323803840867424No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22710.19303788591639445No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21760.18496276519333962No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCAC21620.18377274740257368No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21380.1817327169041177No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20380.17323258982721793No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19420.1650724678333941No Hit
GTGTTGGTACCAGGAGACATAGTGATAACCACCAACATCACTGCTGGTTC19210.16328744114724514No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATCATTTATGATGTCA18770.15954738523340925No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG18530.15750735473495328No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCACCATCTCCTG18220.15487231534111434No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18120.15402230263342437No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18100.15385230009188636No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATCACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGC17950.1525772810303514No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17710.15053725053189546No Hit
GTAATAATCAGCCTCATCCTCAACCTGGAGCCCAGAGATGGTCAGGGAGG17350.14747720478421153No Hit
TCTCTGGGCTCCAGGTTGAGGATGAGGCTGATTATTACTGCTGCTCATAT17240.14654219080575254No Hit
CTATGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCATCA16820.14297213743345463No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT16800.14280213489191665No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16790.14271713362114763No Hit
GTGTAGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAAC16590.14101710820576768No Hit
GTCTGATAACTGAGGACACCGCCTTCTATTACTGTGCAAAAGATGGTGAC16410.13948708533192572No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC16350.1389770777073117No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16200.13770205864577675No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15620.13277198494117487No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15520.1319219722334849No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15480.1315819671504089No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15450.1313269633381019No Hit
CTCCTGTGCAGCCTCTGGATTCAACTTTGATGATTATACCATGTACTGGGTCCGTCAAGCTCCGGGGAAGGGT15400.1309019569842569No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15050.127926912507342No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15030.127756909965804No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC15020.12767190869503497No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14680.12478186548888906No Hit
GTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGACATAGTGATAA14370.12214682609505013No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14140.12019179686736316No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14020.1191717816181352No Hit
TTCTATTACTGTGCAAAAGATGGTGACGGTCTGGGGAACACCAACTGGTACTTCGATTTCTGGGGCCGTGGCACC14020.1191717816181352No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13940.11849177145198321No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13790.11721675239044824No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13790.11721675239044824No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13680.11628173841198926No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13500.1147517155381473No Hit
CCCCTGAGGGCCGCTTACTGACATCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA13170.11194667360277036No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCACCATCTCCTGCA12860.10931163420893142No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC12570.10684659735663048No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12530.1065065922735545No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCACCATCT12440.10574158083663351No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12260.10421155796279155No Hit
CAGCTACACTTTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA12170.10344654652587056No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAATCAGTCAC12170.10344654652587056No Hit
CTCCAGACCCTTCCCCGGAGCTTGACGGACCCAGTACATGGTATAATCAT12110.10293653890125658No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11850.10072650586126262No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11840.10064150459049362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)