FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141788
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGGTACCTCACTCTGGACGTTCGGCCAGGGGA54980.48152546707444815No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37090.3248413891195213No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGAGCTCAACTTCAGGGAGA32410.2838530445231514No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA31990.2801746033414259No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29980.26257063482888243No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC25240.22105679863512318No Hit
GTCTCTAGTGGCTCCGTCAGGAGTGGTAGTTACTACTGGAGCTGGGTCCG23880.2091456557609644No Hit
GTGTATTACTGTCAGCACTATGGTACCTCACTCTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA23020.20161360953171692No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22430.19644627549072158No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22100.1935560717050801No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGACAGCTCCCAGATGGGTC20640.18076910950193908No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20260.17744099605180647No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19890.1742004645345721No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19800.17341222713848806No Hit
CTATGGTACCTCACTCTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19660.1721860800779129No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19570.17139784268182884No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19170.1678945653658998No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18740.16412854225127607No Hit
GGGTATATCTCTAGCAGTGGGGTTCCCTACTACAACCCCTCCCTGAACAGTCGAGTCACCATATCACAAGACAC18700.16377821451968316No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18190.15931153594187364No Hit
ATGGTACCTCACTCTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAA18080.15834813467999312No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17620.1543193657666747No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCGACTACTTAGCCTGGTACC17610.1542317838337765No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17190.150553342652051No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16940.14836379432959534No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16850.1475755569335113No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAG16550.1449480989465645No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15710.1375912165831135No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15650.13706572498572414No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15600.136627815321233No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14960.13102257161574654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14830.1298840064880696No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14620.12804478589720683No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14420.12629314723924231No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14410.12620556530634408No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14250.12480425437997246No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14220.12454150858127779No Hit
GTACCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC14000.1226147060575168No Hit
GTTCAGGGAGGGGTTGTAGTAGGGAACCCCACTGCTAGAGATATACCCAATCCACTCCAGTCTCTTCCCTGGGGG13850.12130097706404341No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13840.1212133951311452No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13790.12077548546665405No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC13600.11911142874158775No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13440.11771011781521612No Hit
GTTTATTACTGTGCGAGAGGAGGGGTGACTGAGTTTCCCCTTCCCTACTG13310.1165715526875392No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13090.11464475016377822No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13010.1139440947005924No Hit
GGACTGGTGAGGCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTCTAGTGGCTCCGTCAGGAGTGGTAGTTA12980.11368134890189772No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12980.11368134890189772No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC12910.11306827537161016No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12730.11149180057944207No Hit
GTCCCTCACCTGCGCTGTCTCTAGTGGCTCCGTCAGGAGTGGTAGTTACT12660.1108787270491545No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12470.10921467032408817No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12440.10895192452539351No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12420.10877676065959706No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12380.10842643292800415No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12190.10676237620293785No Hit
GTCAGAGTGTTATCAGCGACTACTTAGCCTGGTACCGGCAGCAACCTGGC12160.10649963040424318No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGGGTGCCACGG12120.10614930267265027No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTATGGTACCTCA12080.10579897494105736No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11930.10448524594758397No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11890.10413491821599107No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11700.10247086149092477No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11660.10212053375933186No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11630.10185778796063719No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11610.10168262409484072No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11450.10028131316846911No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAG100.0074914712142.3528370
CGCTTAG306.887457E-445.19177219
GGTATCA9300.041.552731
TTGTGAT12100.040.58820770
TGGTATC2750.039.4349142
CTTATAC7050.038.946961
GTATCAA22750.038.4428861
TCTTATA2700.037.654862
TTATACA7450.036.846172
GTGGTAT2700.035.1537741
GCGGGTT501.7437094E-434.88549447
AACTGTG25300.034.88488470
CACGAGA7600.034.79339221
TATACAC7900.034.7473343
TTGCCGT501.7927885E-434.72113844
ATCTCCG7850.034.5354310
CTCTTAT2850.034.492991
CGAGACT7700.034.34754623
ACGAGAC7700.034.3445422
TAAACCG502.0673126E-433.8893785
Produced by FastQC (version 0.11.5)