FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393766
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58790.4218068169262272No Hit
GTAGTAGACAGCCGTGTCTGGGGCGGTCACAGAGTTCACCTTCAGGGAGA44580.3198528303890323No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43040.3088036298776122No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34950.25075945316502196No Hit
GACTATTACTGTCAATCAACTGACAGCAGTGCTACTTATGTGGTGTTCGG34560.24796127900953246No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32580.233755164066278No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32490.2331094315688573No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCAC31810.22823056381056792No Hit
CTATTACTGTCAATCAACTGACAGCAGTGCTACTTATGTGGTGTTCGGCG31010.22249071938905096No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCACATAAGTAGCA30510.21890331662560283No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30080.21581815024903753No Hit
GTCTACTACTGTGCGAGACATACCGTGACAGACTACGGTGCTAAGTCCTGGTTCGACCCCTGGGGCCAGGGAGCC28100.20161203530578306No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27510.1973789000449143No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27100.19443722977888683No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25690.1843207539859632No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25280.18137908371993577No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24940.17893964984079105No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24750.17757643679068078No Hit
CCACATAAGTAGCACTGCTGTCAGTTGATTGACAGTAATAGTCAGCCTCG24600.17650021596164636No Hit
CAGTCACATTGACCATCAGTGGGGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAACTGACAGCAGTG24240.17391728597196374No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23940.17176484431389488No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23160.16616849600291583No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22700.1628680854605436No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22480.16128962824462642No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22370.16050039963666785No Hit
GTCTCTGGTGGCTCCATCGGCGGGAGTACTTACTACTGGGCCTGGATCCG22300.1599981632497851No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22190.15920893464182653No Hit
GTAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCAGATTGCTTT21690.15562153187837843No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21510.15433006688353712No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20080.14407009498007556No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAATGTCCCCAGGACAGACGGCCACGATCAC20000.14349611053792388No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19850.14241988970888944No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC19710.14141541693512397No Hit
GTTCTCTGGCTCCAGCTCAGGGACAACAGTCACATTGACCATCAGTGGGG19650.1409849286035102No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACCCCACTGATGGTCAATGTGA19260.1381867544480207No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18800.13488634390564844No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18520.1328773983581175No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17910.12850076698671084No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17890.1283572708761729No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17670.12677881366025573No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17350.12448287589164897No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17270.12390889144949725No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAATGTCCCCAGGACAGACGGCCACGATCACCTGCTCTGGAGATG16930.12146945757035256No Hit
GTGCTACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA16830.12075197701766292No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC16430.11788205480690445No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16410.11773855869636654No Hit
GTGTCAATGTCCCCAGGACAGACGGCCACGATCACCTGCTCTGGAGATGC16350.11730807036475276No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCACATAAGTAGCAC16250.11659058981206315No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16200.11623184953571833No Hit
CCCCAGGACAGACGGCCACGATCACCTGCTCTGGAGATGCTTTGCCAAAG16180.11608835342518042No Hit
CTTATGTGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCC16120.11565786509356664No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16050.1151556287066839No Hit
ACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT16030.115012132596146No Hit
AAGTAGCACTGCTGTCAGTTGATTGACAGTAATAGTCAGCCTCGTCTTCT15810.11343367538022883No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAATGTCCCCAGGACAGACGG15800.11336192732495985No Hit
ACTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG15750.11300318704861505No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15410.11056375316947034No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15410.11056375316947034No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15360.11020501289312552No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT15350.11013326483785657No Hit
ATGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAACCGCTCAGGGATCCCT15260.10948753234043593No Hit
CACTAAGAGGCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCCAGCTCAG15200.10905704400882214No Hit
CTATTATACTGGAGTCGCCTACTACAACCCGCCCCTCAAGAGTCGAGTCACCATATCCGTTGACACGTTCAAGAA15190.10898529595355319No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14790.1061153737427947No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14650.10511090096902924No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14430.10353244375311207No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14130.10138000209504322No Hit
GACCATCAGTGGGGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAAT14020.10059077348708463No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14020.10059077348708463No Hit
GCCTGGTCCCTCCGCCGAACACCACATAAGTAGCACTGCTGTCAGTTGATTGACAGTAATAGTCAGCCTCGTCTT13970.10023203321073981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAG200.00653558553.32695858
GGTATCA7300.047.519011
AACGGAT14700.044.02911470
GTATCAA20950.041.881841
CCGCTAT700.001160804640.6422670
TATACAC4200.040.4705733
ATCTCGT3150.040.29234737
CGTATGC3650.039.69316541
TCGTATG3650.039.62968440
TTATACA4250.039.194562
CTTATAC4300.038.7541051
CGAGACT3800.037.5818223
ACTAGGC4000.037.44585827
TAGGCAT4100.037.4031429
GATCTCG3400.037.29187436
GACTAGG3950.037.03941326
ACGAGAC3800.036.68570322
CACGAGA3800.036.68438721
AGGGGGG4850.036.66183570
TACACAT4650.035.8229875
Produced by FastQC (version 0.11.5)