FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393766
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54230.38908970372358054No Hit
GTAGTAGACAGCCGTGTCTGGGGCGGTCACAGAGTTCACCTTCAGGGAGA42840.3073686687722329No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42470.30471399072728134No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40460.29029263161822No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36740.26360235505816615No Hit
GACTATTACTGTCAATCAACTGACAGCAGTGCTACTTATGTGGTGTTCGG33510.24042773320629143No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31010.22249071938905096No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30250.21703786718860985No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29300.21022180193805848No Hit
CTATTACTGTCAATCAACTGACAGCAGTGCTACTTATGTGGTGTTCGGCG29040.20835635250106543No Hit
GTCTACTACTGTGCGAGACATACCGTGACAGACTACGGTGCTAAGTCCTGGTTCGACCCCTGGGGCCAGGGAGCC28550.20484069779288633No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27810.19953134170298314No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27640.19831162476341077No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCAC27310.19594393893953505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26910.19307401672877658No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26380.18927136979952158No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCACATAAGTAGCA25410.18231180843843228No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24930.17886790178552212No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24330.1745630184693844No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23530.16882317404786742No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23170.1662402440581848No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22670.1626528412947367No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22550.16179186463150916No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22530.16164836852097125No Hit
GTTCTCTGGCTCCAGCTCAGGGACAACAGTCACATTGACCATCAGTGGGG22490.16136137629989541No Hit
CAGTCACATTGACCATCAGTGGGGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAACTGACAGCAGTG22160.15899369047601966No Hit
GTCTCTGGTGGCTCCATCGGCGGGAGTACTTACTACTGGGCCTGGATCCG21180.1519623810596614No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20470.14686826913556508No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAATGTCCCCAGGACAGACGGCCACGATCAC20390.14629428469341338No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20300.14564855219599274No Hit
GTAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCAGATTGCTTT20130.14442883525642036No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20060.14392659886953763No Hit
CCACATAAGTAGCACTGCTGTCAGTTGATTGACAGTAATAGTCAGCCTCG20020.1436396066484618No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19420.13933472333232408No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC19210.13782801417167587No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18170.13036621642370383No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18100.1298639800368211No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17450.12520035644433858No Hit
GTGCTACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17310.1241958836705731No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAATGTCCCCAGGACAGACGGCCACGATCACCTGCTCTGGAGATG17150.12304791478626971No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16920.1213977095150836No Hit
CTTATGTGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCC16680.1196757561886285No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16450.11802555091744238No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAATGTCCCCAGGACAGACGG16230.11644709370152521No Hit
GTGTCAATGTCCCCAGGACAGACGGCCACGATCACCTGCTCTGGAGATGC16160.11594485731464249No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16090.11544262092775975No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15970.11458164426453221No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15710.1127161948275392No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC15700.11264444677227024No Hit
CACTAAGAGGCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCCAGCTCAG15640.11221395844065647No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15550.11156822594323582No Hit
CCCCAGGACAGACGGCCACGATCACCTGCTCTGGAGATGCTTTGCCAAAG15540.11149647788796684No Hit
CTATTATACTGGAGTCGCCTACTACAACCCGCCCCTCAAGAGTCGAGTCACCATATCCGTTGACACGTTCAAGAA15470.11099424150108411No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15460.11092249344581515No Hit
ACTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG15450.1108507453905462No Hit
ACTTATGTGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT15360.11020501289312552No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15280.10963102845097383No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15190.10898529595355319No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15130.1085548076219394No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG15060.10805257123505667No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14780.10604362568752575No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACCCCACTGATGGTCAATGTGA14770.10597187763225677No Hit
ATGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAACCGCTCAGGGATCCCT14350.10295845931096038No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13960.10016028515547086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT301.7071165E-595.40195570
GTACGTA355.7980287E-875.9917869
GGTACGT402.4228424E-548.75628368
AACGGAT14200.046.3572970
GTGGTAT2950.043.7732621
GGTATCA10050.043.6185261
TGGTACG456.441793E-541.31511767
TGGTATC3450.036.452182
GTATCAA22700.034.7303731
CAACGGA13950.031.77673569
GCTTGTA751.878896E-631.7164151
ATCAACG25750.031.0159233
TATCAAC25900.030.4459192
TCAACGC26200.030.355674
GGTCTAG450.004965925630.22129420
GGAACCG450.00497552630.209365
CAACGCA26600.029.8970495
AACGCAG27300.029.2538956
AATGGTA4250.028.62058670
ACGCAGA28350.028.2902937
Produced by FastQC (version 0.11.5)