FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826944
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT53970.6526439517065242No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24770.2995366070737559No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23530.2845416376441452No Hit
ATATTATATCACCCCGCTCACTTTTGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT18950.2291569924928411No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17110.2069063926940639No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16580.20049725253463352No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16490.1994089079792586No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16440.19880427211516136No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15220.18405115703118954No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15200.18380930268555065No Hit
CTTTAGGAGGCTGTCCTGGTTTATGTTGATACCAAGCCAAGTAGTTCCTA14850.1795768516368702No Hit
GTCCCAGACCGATTCAGTGGCGGCGGGTCTGGGACAGATTTCAATCTCAC14700.1777629440445786No Hit
CTCCTAAAGTGCTCATCTACTGGGCATCTACCCGGGCATTCGGAGTCCCA14690.17764201687175915No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13900.1680887702190233No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC13680.1654283724169956No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12810.1549077083817042No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12640.1528519464437737No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12510.15127989319712098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12420.150191548641746No Hit
ATATCACCCCGCTCACTTTTGGCGGAGGGACCAAGGTAGAGATCAAACGA12370.1495869127776488No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12290.14861949539509325No Hit
ACATAAACCAGGACAGCCTCCTAAAGTGCTCATCTACTGGGCATCTACCCGGGCATTCGGAGTCCCAGACCGATT12140.14680558780280165No Hit
CTTTATTACTGTCAACAATATTATATCACCCCGCTCACTTTTGGCGGAGGGACCAAGGTAGAGATCAAACGAA10950.13241525423728812No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10900.13181061837319094No Hit
GTAATAAAGTGCCACATCTTCAGCCTGCACGTTGCTGATGGTGAGATTGAAATCTGTCCCAGACCCGCCGCCA10860.13132690968191316No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10650.1287874390527049No Hit
GTCTGGGACTCCGAATGCCCGGGTAGATGCCCAGTAGATGAGCACTTTAGGAGGCTGTCCTGGTTTATGTTGATA10340.12503869669530224No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10100.12213644454763563No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9550.11548545004256637No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9090.10992280009287207No Hit
CTTTTGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCA9080.10980187292005263No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8960.10835074684621933No Hit
GTCCCAGACCCGCCGCCACTGAATCGGTCTGGGACTCCGAATGCCCGGGT8830.1067786935995666No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8810.1065368392539277No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8730.1055694218713722No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8580.10375551427908057No Hit
GACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT8490.10266716972370558No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8480.10254624255088617No Hit
ATATAATATTGTTGACAGTAATAAAGTGCCACATCTTCAGCCTGCACGTTGCTGATGGTGAGATTGAAATCTGTC8380.10133697082269175No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8270.1000067719216779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)