FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826944
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109801.3277803575574647No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23820.288048525655909No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20480.2476588499342156No Hit
ATATTATATCACCCCGCTCACTTTTGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT20210.2443938162680907No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18840.22782679359182725No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17760.2147666589273276No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15770.1907021515362588No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13990.16917711477439829No Hit
CTCCTAAAGTGCTCATCTACTGGGCATCTACCCGGGCATTCGGAGTCCCA13740.16615393545391224No Hit
GTCCCAGACCGATTCAGTGGCGGCGGGTCTGGGACAGATTTCAATCTCAC13570.16409817351598172No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13280.16059128550421795No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13080.1581727420478291No Hit
CTTTAGGAGGCTGTCCTGGTTTATGTTGATACCAAGCCAAGTAGTTCCTA12920.15623790728271805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12590.1522473105796765No Hit
ATATCACCCCGCTCACTTTTGGCGGAGGGACCAAGGTAGAGATCAAACGA12440.1504334029873849No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12250.1481357867038155No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12030.14547538890178777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11610.14039644764337125No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11580.14003366612491294No Hit
ACATAAACCAGGACAGCCTCCTAAAGTGCTCATCTACTGGGCATCTACCCGGGCATTCGGAGTCCCAGACCGATT11320.13688955963160745No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11230.1358012150762325No Hit
GTAATAAAGTGCCACATCTTCAGCCTGCACGTTGCTGATGGTGAGATTGAAATCTGTCCCAGACCCGCCGCCA10730.12975485643526044No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10640.12866651187988545No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10560.1276990944973299No Hit
CTTTATTACTGTCAACAATATTATATCACCCCGCTCACTTTTGGCGGAGGGACCAAGGTAGAGATCAAACGAA10480.1267316771147744No Hit
GGGTGATATAATATTGTTGACAGTAATAAAGTGCCACATCTTCAGCCTGCACGTTGCTGATGGTGAGATTGAAAT10470.12661074994195495No Hit
GTCTGGGACTCCGAATGCCCGGGTAGATGCCCAGTAGATGAGCACTTTAGGAGGCTGTCCTGGTTTATGTTGATA10180.12310386193019117No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10000.12092717281944121No Hit
ATATAATATTGTTGACAGTAATAAAGTGCCACATCTTCAGCCTGCACGTTGCTGATGGTGAGATTGAAATCTGTC9590.11596915873384414No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9200.11125299899388594No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9140.11052743595696926No Hit
GTCCCAGACCCGCCGCCACTGAATCGGTCTGGGACTCCGAATGCCCGGGT8390.10145789799551118No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8390.10145789799551118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)