FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513836
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGAACCAACAATAAGGACTACTTAGCTTGGTACCAACAGAA67690.4471422267669682No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65190.43062788835778776No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53960.35644548022374944No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43750.28900092216065676No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42570.28120615443152364No Hit
GATTACACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTACT39160.2586805968414016No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38510.2543868688550147No Hit
ATATTATGGTACTCCTTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT37730.2492343952713504No Hit
CTCCTAAGCTACTCATTTACTGGGGATCTACTCGGGATTCCGGAGTCCCT35630.23536235100763886No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35550.2348338921785451No Hit
CCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGTAATCTGTC33340.22023521702482962No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33250.21964070084209913No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33100.2186498405375483No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31520.2082127786629463No Hit
GTCCCTGACCGATTCAATGGCAGCGGGTCTGGGACAGATTACACTCTCAC30880.20398510803019612No Hit
GTATAAAAGACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAACCAGGGAGTCTGGAGA29680.19605822559378958No Hit
GTTTATTACTGTCAGCAATATTATGGTACTCCTTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAA29190.19282141526559018No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28690.1895185475837541No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27130.17921360041642553No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27090.17894937100187866No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27070.17881725629460524No Hit
GTGTAAGGAGTACCATAATATTGCTGACAGTAATAAACTGCCACATCTTC26730.1765713062709567No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTG26580.17558044596640587No Hit
GCTTAGGAGGCTGTCCTGCTTTCTGTTGGTACCAAGCTAAGTAGTCCTTA26350.17406112683276126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26090.1723436356382065No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGT26030.17194729151638619No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25650.1694371120781908No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCC25640.16937105472455405No Hit
CAATAATAAAGGGCCGTGTCCGCGGCTTCCAAGTTGTTCATTTGCAGATA25490.1683801944200032No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25450.16811596500545634No Hit
CCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC24600.162501089946335No Hit
ACTTAGCTTGGTACCAACAGAAAGCAGGACAGCCTCCTAAGCTACTCATT23700.15655592811903007No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22800.15061076629172512No Hit
GTCAGGGACTCCGGAATCCCGAGTAGATCCCCAGTAAATGAGTAGCTTAGGAGGCTGTCCTGCTTTCTGTTGGTA22490.14856298832898676No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22170.14644915301261166No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22160.14638309565897495No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21630.1428820559162287No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21590.1426178265016818No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21480.14189119561167787No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21410.14142879413622084No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC21100.13938101617348247No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20520.1355496896625526No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20490.13535151760164246No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20450.13508728818709556No Hit
GTCCTAGTAATGCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20410.13482305877254866No Hit
CTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC20400.13475700141891195No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20350.13442671465072834No Hit
CCTTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20290.134030370528908No Hit
GTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTTTTATA19810.1308596175543454No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19640.12973664254252112No Hit
GTCTGGGACAGATTACACTCTCACCATCACCAGCCTGCAGGCTGAAGATG19450.1284815528234234No Hit
GGACTACTTAGCTTGGTACCAACAGAAAGCAGGACAGCCTCCTAAGCTAC19150.12649983221432176No Hit
CAATAAGGACTACTTAGCTTGGTACCAACAGAAAGCAGGACAGCCTCCTAAGCTACTCATTTACTGGGGATCTA18310.12095101450883715No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18230.12042255567974339No Hit
GGTCCTAGTAATGCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAG18220.12035649832610666No Hit
GAGTACCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGTAAT18110.11962986743610272No Hit
GTACCAACAGAAAGCAGGACAGCCTCCTAAGCTACTCATTTACTGGGGATCTACTCGGGATTCCGGAGTCCCTGA17830.11778026153427452No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17750.11725180270518074No Hit
ATTCAATGGCAGCGGGTCTGGGACAGATTACACTCTCACCATCACCAGCC17620.11639305710790337No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17600.11626094240062992No Hit
CTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTTTTATACAGAACCAA17510.11566642621789942No Hit
GGATTCCGGAGTCCCTGACCGATTCAATGGCAGCGGGTCTGGGACAGATT17360.1146755659133486No Hit
CTACTTAGCTTGGTACCAACAGAAAGCAGGACAGCCTCCTAAGCTACTCATTTACTGGGGATCTACTCGGGATTC17160.11335441884061419No Hit
AAGCAGGACAGCCTCCTAAGCTACTCATTTACTGGGGATCTACTCGGGAT17120.11309018942606729No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA17080.1128259600115204No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17030.1124956732433368No Hit
GGCCAAAAGTGTAAGGAGTACCATAATATTGCTGACAGTAATAAACTGCC17010.11236355853606335No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGT16730.11051395263423514No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16730.11051395263423514No Hit
GGATCTCTGGTGCCTACGGGAAGGTCCTAGTAATGCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGG16550.10932492026877415No Hit
TTATTATTGTGTCCGAAATTTAGGTTGGGGCTGGATGGACCCCTGGGGCC16480.10886251879331711No Hit
ATGGTACTCCTTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGA16290.1076074290742194No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16240.1072771423060358No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16200.10701291289148891No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16070.10615416729421152No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15840.10463484816056692No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15820.10450273345329347No Hit
AGATTACACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTA15810.10443667609965678No Hit
TAGTAATGCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCC15710.10377610256328955No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT15710.10377610256328955No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15650.10337975844146921No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15480.10225678342964495No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15290.10100169371054725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTA208.215763E-569.5002545
CTAACGC200.00791274150.8097953
ATGCCGT3600.045.3022144
CTTATAC5400.045.1911281
TATACAC5500.044.343093
CGTATGC3700.043.8522941
CGTAGAG5500.043.8329128
ACGTAGA5600.043.62618327
CGAGACG5550.043.3461623
GAGACGT5650.043.18723724
TCGTATG3600.043.0771140
GACGTAG5600.043.00461226
AGACGTA5650.042.602925
GTCGTAT503.917081E-641.28604539
TCTCCGA5750.041.2355711
TAGAGGA5800.041.04004330
AATCGCG504.0835166E-641.03685436
TACACAT5900.040.762665
ATCTCCG5900.040.76131410
GTATGCC4050.040.1450242
Produced by FastQC (version 0.11.5)