FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513836
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65560.43307201044234644No Hit
CTTTTATACAGAACCAACAATAAGGACTACTTAGCTTGGTACCAACAGAA58740.38802089526210237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56080.3704496391947344No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43980.2905202412943014No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41830.2763179102624062No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40090.2648239307296167No Hit
ATATTATGGTACTCCTTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT38730.2558401306350226No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37760.24943256733226055No Hit
GATTACACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTACT37710.24910228056407696No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36940.24401586433404943No Hit
CTCCTAAGCTACTCATTTACTGGGGATCTACTCGGGATTCCGGAGTCCCT33240.21957464348846242No Hit
CCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGTAATCTGTC32560.21508274344116535No Hit
GTCCCAGACCCGCTGCCATTGAATCGGTCAGGGACTCCGGAATCCCGAGT30770.20325847714019218No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30510.20154098594563744No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29830.19704908589834036No Hit
GTTTATTACTGTCAGCAATATTATGGTACTCCTTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAA28910.19097180936376199No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27820.18377155781735935No Hit
GTCCCTGACCGATTCAATGGCAGCGGGTCTGGGACAGATTACACTCTCAC27540.18192195191553115No Hit
GTGTAAGGAGTACCATAATATTGCTGACAGTAATAAACTGCCACATCTTC27250.1800062886600662No Hit
GAGTGTAATCTGTCCCAGACCCGCTGCCATTGAATCGGTCAGGGACTCCG27070.17881725629460524No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGT27020.17848696952642162No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTG26690.1763070768564098No Hit
GTATAAAAGACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAACCAGGGAGTCTGGAGA25780.17029585767546815No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25620.1692389400172806No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25430.1679838502981829No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25220.16659664587181175No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25100.16580395762817107No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCC25060.1655397282136242No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25010.16520944144544059No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25000.16514338409180387No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24730.16335983554361239No Hit
GCTTAGGAGGCTGTCCTGCTTTCTGTTGGTACCAAGCTAAGTAGTCCTTA24020.15866976343540515No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23820.15734861636267072No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23750.15688621488721366No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23340.15417786338810807No Hit
CCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC23000.15193191336445955No Hit
CAATAATAAAGGGCCGTGTCCGCGGCTTCCAAGTTGTTCATTTGCAGATA22960.15166768394991267No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20750.1370690087961972No Hit
GTCAGGGACTCCGGAATCCCGAGTAGATCCCCAGTAAATGAGTAGCTTAGGAGGCTGTCCTGCTTTCTGTTGGTA20650.13640843525982999No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20330.1342945999434549No Hit
ACTTAGCTTGGTACCAACAGAAAGCAGGACAGCCTCCTAAGCTACTCATT20050.1324449940416267No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20020.13224682198071652No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19770.1305953881397985No Hit
GTCCTAGTAATGCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC19660.12986875724979455No Hit
GTCTGGGACAGATTACACTCTCACCATCACCAGCCTGCAGGCTGAAGATG19540.1290760690061539No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19460.12854761017706012No Hit
CCTTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19330.12768886457978273No Hit
GTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTTTTATA18530.12240427628884501No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18470.12200793216702471No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC18430.12174370275247781No Hit
GTGTAATCTGTCCCAGACCCGCTGCCATTGAATCGGTCAGGGACTCCGGA18410.12161158804520437No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18400.12154553069156765No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18390.12147947333793092No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18360.12128130127702075No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18230.12042255567974339No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGT18150.1198940968506496No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18150.1198940968506496No Hit
GGACTACTTAGCTTGGTACCAACAGAAAGCAGGACAGCCTCCTAAGCTAC18100.119563810082466No Hit
GAGTACCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGTAAT17870.1180444909488214No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGTAATCTGTCCCAGACCCGCTGCCATTGAATCGGTCAGGGACT17830.11778026153427452No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17800.11758208947336435No Hit
GGTCCTAGTAATGCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAG17750.11725180270518074No Hit
GGCCAAAAGTGTAAGGAGTACCATAATATTGCTGACAGTAATAAACTGCC17430.11513796738880566No Hit
ATTCAATGGCAGCGGGTCTGGGACAGATTACACTCTCACCATCACCAGCC17330.11447739385243845No Hit
CTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC17250.11394893502334466No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA16510.10906069085422727No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16450.10866434673240694No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16430.10853223202513351No Hit
ATGGTACTCCTTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGA16260.10740925701330925No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16180.10688079818421546No Hit
CTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTTTTATACAGAACCAA15940.10529542169693414No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15810.10443667609965678No Hit
GGATTCCGGAGTCCCTGACCGATTCAATGGCAGCGGGTCTGGGACAGATT15760.10410638933147315No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTACTCCTTACACTTTTGGCCAGGGGACCAGG15690.10364398785601611No Hit
AGATTACACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGGTA15570.10285129961237545No Hit
GTACCAACAGAAAGCAGGACAGCCTCCTAAGCTACTCATTTACTGGGGATCTACTCGGGATTCCGGAGTCCCTGA15520.10252101284419185No Hit
GGATCTCTGGTGCCTACGGGAAGGTCCTAGTAATGCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGG15340.10133198047873086No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15220.1005392922350902No Hit
CTACTTAGCTTGGTACCAACAGAAAGCAGGACAGCCTCCTAAGCTACTCATTTACTGGGGATCTACTCGGGATTC15170.10020900546690659No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15140.10001083340599641No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTA259.4676495E-486.8095970
GGTATCA11150.041.546791
GTGGTAT3100.037.0859151
TGGTATC3300.034.8325272
GTATCAA23900.033.5306661
CAGTATC751.9532472E-631.5593851
ACAGGTA1950.031.212581
ATAAACG652.6656275E-531.2094863
TAAACGC553.666209E-430.7376294
GACAGGT2150.029.881811
TATCAAC28450.028.1634652
ATCAACG28400.028.0958613
CATTCGA755.775036E-527.86582846
AACGCAG29200.027.6743956
CAACGCA28800.027.5891595
TCAACGC28700.027.5674764
GCCTGAC4950.027.3223387
ACACGGT500.00853521127.0491166
TATAAAC757.076624E-527.0464342
TCGTATA1755.456968E-1226.80520261
Produced by FastQC (version 0.11.5)