FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772477
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA48040.6218955386373963No Hit
CTCCTAAACTGCTCGTTTATTGGGCATCTACCCGGGAATCCGGGGTCCCT37760.488817142775772No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30130.3900439754193329No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30110.38978506803438806No Hit
GTTTAGGAGGCTGTCCTTGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT29490.3817589391010995No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA24470.31677318547995603No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21210.27457128173395456No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19980.2586484775598497No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19530.2528230613985918No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19110.2473860063147511No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18590.24065441430618645No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17220.22291925843746802No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16670.21579930535148617No Hit
ATTATAATATTGCTGACAGTAGTAAACTGCCACATCTTCAGCACGCAGGCTGCTGATGGTGAGAGTGAAAT16400.21230405565473148No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15670.20285393610424649No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15670.20285393610424649No Hit
GTTTACTACTGTCAGCAATATTATAATAGTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA14950.19353327024623387No Hit
ATATTATAATAGTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT14630.18939075208711714No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14410.18654277085272442No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13790.1785166419194358No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13390.1733384942205399No Hit
GATTTCACTCTCACCATCAGCAGCCTGCGTGCTGAAGATGTGGCAGTTTACTACTGTCAGCAATATTATAATAGT12310.15935749543352098No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12180.15767459743137982No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11780.15249644973248394No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11090.14356414495188852No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA11000.14239906171963695No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10960.14188124694974735No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC10950.14175179325727497No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA10930.1414928858723302No Hit
GTAGTAAACTGCCACATCTTCAGCACGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10820.1400688952551338No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10760.13929217310029943No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10580.13696200663579627No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10420.13489074755623792No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC10060.13023041462723162No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10050.13010096093475923No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9760.1263468038530597No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9650.12492281323586334No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG9460.1224631930788878No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9440.122204285693943No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9240.11961521184449504No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9220.11935630445955026No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9150.11845012861224347No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9050.1171555916875195No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9040.1170261379950471No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT9000.11650832322515751No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8990.11637886953268513No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGGACTATT8920.11547269368537834No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8880.11495487891548875No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAATAAACGAGCAGTTTAGGAGGCTGTCCTTGTTTCTGCTGGTA8880.11495487891548875No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8840.11443706414559916No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8690.11249525875851321No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCGTGCTGAAGATGTGGCAGTTTA8690.11249525875851321No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8660.11210689768109601No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8620.11158908291120642No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8610.11145962921873401No Hit
CTACTTAGCTTGGTACCAGCAGAAACAAGGACAGCCTCCTAAACTGCTCGTTTATTGGGCATCTACCCGGGAATC8540.11055345337142725No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8520.11029454598648246No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8380.10848219429186888No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8280.10718765736714492No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8080.10459858351769696No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8060.10433967613275216No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7900.10226841705319382No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7860.10175060228330424No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7830.10136224120588704No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA7770.10058551905105266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTC150.002237829469.89545450
TATCGTC200.00775467151.06744417
TATCTCG1800.045.74572436
GTGGTAT2200.043.346791
GGTATCA7150.042.394111
CGTATGC2300.042.00076341
GCCTATC2100.040.75133533
TAGGCAA857.417305E-540.32966670
GTATCAA19100.039.9425931
TACACAT2800.038.9009745
TGGTATC2450.038.9009742
GAGCCTA2550.038.8713131
CGTCTTC2450.038.39135748
ATGCCGT2450.038.16381544
TCGTATG2350.038.108440
CTATCTC2250.038.08664735
CCGTCTT2500.037.5613247
GGTCTGG16150.037.35800670
GTATGCC2600.037.1984542
CCTATCT2250.036.53460334
Produced by FastQC (version 0.11.5)