FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805493
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCGTCGCCTCTCAGGCTGTTCATTTGCAAATAGA30840.38287111123249984No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25960.3222870962255414No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25690.31893511178868095No Hit
GATTATTACTGCTGCTCAAATGTAGGTTATAGCGCTTATGTCTTCGGAATTGGGACCAAGGTCACCGTCCTAGGT22120.27461442867908226No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22010.27324880538999097No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21100.26195137636205407No Hit
GTTTATTACTGTGCGAGAGGGGCTAGTGAGTGGGACCCGATTGACCACTGGGGCCAGGGAACCATGGTCAGCGT20840.2587235394969292No Hit
GACCTAGGACGGTGACCTTGGTCCCAATTCCGAAGACATAAGCGCTATAACCTACATTTGAGCAGCAGTAATAAT19430.24121873188221377No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19300.23960481344965134No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCAGAGATATT18610.23103863099989694No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18600.23091448342816137No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCTCCTG17920.2224724485501426No Hit
CTCTTGTGCAGCGTATGGATTTACGTTTAGTAGCTATGGCATGCACTGGGTCCGCCAGGCCCCGGGCAAGGGA17100.21229234766782576No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16130.20025003320947543No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16050.19925685263559087No Hit
CTTATAACCTTGTCTCCTGGTTCCAACAACACCCAGGCAAGGCCCCCAAA16000.198636114776913No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC15210.18882845660980294No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGGCCAGAGATTGTCAGGGAGG15170.18833186632286064No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAATTCCGAAGACATAAGCGCTATAACCTACATTTGAGCAG14820.1839867013121157No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCAC14430.17914494601442843No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14310.17765517515360157No Hit
ACCTAGGACGGTGACCTTGGTCCCAATTCCGAAGACATAAGCGCTATAAC14140.17554466643409689No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13850.17194438685376534No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13770.17095120627988075No Hit
CTCCTGCACTGGAACCAGCAGAGATATTGGGACTTATAACCTTGTCTCCTGGTTCCAACAACACCCAGGCAAGGC13710.17020632084946735No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13700.17008217327773176No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13630.16921314027558276No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13460.16710263155607807No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12840.1594054821084727No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12340.15319810352169416No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12310.15282566080648743No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12040.14947367636962705No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11850.1471148725066512No Hit
GGACTGGAGTGGCTGGCAGTTATTTGGTTTGATGGCAGGAATAAAAATTA11770.14612169193276664No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCT11630.14438362592846865No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11470.14239726478069953No Hit
CTCCAGTCCCTTGCCCGGGGCCTGGCGGACCCAGTGCATGCCATAGCTAC10700.13283790175706056No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10640.13209301632664716No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10600.13159642603970487No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10490.13023080275061358No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10470.12998250760714247No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAATGAGGAGTTTGGGGGCCTTGCCTGGGTGTTGTTGGAACCAGGAGA10410.12923762217672904No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10220.1268788183137532No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10050.12476830959424849No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9790.12154047272912366No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9710.12054729215523907No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9430.11707116014664312No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9370.11632627471622968No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9170.11384332328151828No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGCCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGCCTCCAGG9150.11359502813804714No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8980.11148451941854244No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8630.10713935440779747No Hit
CACTAGCCCCTCTCGCACAGTAATAAACAGCCGTGTCGTCGCCTCTCAGG8570.10639446897738403No Hit
GTCGGTCACCATCTCCTGCACTGGAACCAGCAGAGATATTGGGACTTATAACCTTGTCTCCTGGTTCCAACAACA8420.10453225540135047No Hit
GCCTGGAGGCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGC8410.10440810782961492No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC8180.10155271367969679No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8160.10130441853622564No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8100.10055953310581221No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8080.10031123796234108No Hit
TCTCTGGCCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCAAAT8080.10031123796234108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)