FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805493
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCGTCGCCTCTCAGGCTGTTCATTTGCAAATAGA27330.33929531355331455No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23090.2866567431374326No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23040.2860360052787547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22950.2849186771331346No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT22300.2768490849703225No Hit
GATTATTACTGCTGCTCAAATGTAGGTTATAGCGCTTATGTCTTCGGAATTGGGACCAAGGTCACCGTCCTAGGT20800.25822694920998696No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20460.2540059317709775No Hit
GTTTATTACTGTGCGAGAGGGGCTAGTGAGTGGGACCCGATTGACCACTGGGGCCAGGGAACCATGGTCAGCGT19580.24308094545824732No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19140.23761845230188222No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCAGAGATATT18800.2333974348628728No Hit
GACCTAGGACGGTGACCTTGGTCCCAATTCCGAAGACATAAGCGCTATAACCTACATTTGAGCAGCAGTAATAAT18120.224955399984854No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCTCCTG16650.20670570693972512No Hit
CTCTTGTGCAGCGTATGGATTTACGTTTAGTAGCTATGGCATGCACTGGGTCCGCCAGGCCCCGGGCAAGGGA15970.19826367206170634No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15470.19205629347492778No Hit
CTTATAACCTTGTCTCCTGGTTCCAACAACACCCAGGCAAGGCCCCCAAA15260.1894491944684808No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15250.18932504689674523No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAATTCCGAAGACATAAGCGCTATAACCTACATTTGAGCAG14720.18274522559475997No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCAC14670.1821244877360821No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGGCCAGAGATTGTCAGGGAGG14080.17479978100368346No Hit
CTCCTGCACTGGAACCAGCAGAGATATTGGGACTTATAACCTTGTCTCCTGGTTCCAACAACACCCAGGCAAGGC13990.1736824528580633No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13790.1711995014233519No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13180.1636264995474821No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12940.1606469578258284No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12790.15878474424979486No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC12560.1559293500998767No Hit
ACCTAGGACGGTGACCTTGGTCCCAATTCCGAAGACATAAGCGCTATAAC12350.15332225109342973No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11890.1476114627935935No Hit
CTCCAGTCCCTTGCCCGGGGCCTGGCGGACCCAGTGCATGCCATAGCTAC11890.1476114627935935No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11480.1425214123524351No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCT11450.14214896963722837No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11440.14202482206549283No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11290.14016260848945924No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10820.13432767261788742No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10640.13209301632664716No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10620.13184472118317603No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10180.1263822280268109No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAATGAGGAGTTTGGGGGCCTTGCCTGGGTGTTGTTGGAACCAGGAGA9940.12340268630515722No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9890.12278194844647936No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9850.12228535815953707No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9800.12166462030085921No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9710.12054729215523907No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9460.11744360286184982No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9250.11483650385540284No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9040.11222940484895585No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9010.11185696213374914No Hit
GTAATAAACAGCCGTGTCGTCGCCTCTCAGGCTGTTCATTTGCAAATAGACCGTATTATTGGAGTTGTCTCTGGA8980.11148451941854244No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8960.1112362242750713No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8950.11111207670333571No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC8810.10937401069903772No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC8750.10862912526862431No Hit
GGACTGGAGTGGCTGGCAGTTATTTGGTTTGATGGCAGGAATAAAAATTA8700.10800838740994646No Hit
GTCGGTCACCATCTCCTGCACTGGAACCAGCAGAGATATTGGGACTTATAACCTTGTCTCCTGGTTCCAACAACA8600.10676691169259075No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGCCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGCCTCCAGG8430.10465640297308604No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8390.10415981268614377No Hit
CCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCAAATGTAGGTT8290.10291833696878805No Hit
CACTAGCCCCTCTCGCACAGTAATAAACAGCCGTGTCGTCGCCTCTCAGG8160.10130441853622564No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8060.10006294281886995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGG150.002403642768.6472435
AACCTAG252.2793353E-456.54258360
TAATGCG305.352323E-447.56237864
GACAGGT1400.041.4430921
ATACGTA350.0014236138.9907311
TACGTAC350.001424044638.9883112
TCTGGAA11100.038.20082570
GTGGTAT1700.038.1448551
GGTATCA5700.037.7221951
GTATCAA12800.037.3292541
GGTACGT450.002520478834.7073868
GTGCTAG400.002736962934.1296041
ATAGTAC400.002738630434.1253663
TATCAAC14650.032.611272
ATCAACG15350.031.3464283
AACGCAG15800.030.8836966
TCAACGC15800.030.4536514
CAACGCA15850.030.357585
GCTTGCC704.1437732E-529.2539481
GACACTC604.9761037E-429.17343754
Produced by FastQC (version 0.11.5)