FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140998
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30350.2659952077041327No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28420.2490801912010363No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24920.21840529080681997No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24450.21428608989673953No Hit
GTCTACATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG22340.19579350708765486No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22020.19298894476589792No Hit
GTCATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGT18480.16196347408146203No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17910.1569678474458325No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17780.15582849400261875No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16990.14890473077078137No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15870.13908876264463216No Hit
CCCTTAGACTCTCCTGTGTAGCCTCTGGTTTCACTTTCATTGACGCCTGG15580.13654712804053995No Hit
GTATGACAGTGCGCCCGCCACTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAACTGTGGCTGCACCAT15510.13593363003265563No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15250.13365492314622812No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15230.1334796380011183No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15160.132866139993234No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14910.1306750756793614No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14800.12971100738125746No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14780.12953572223614765No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14570.12769522821249468No Hit
CTCCTGTGTAGCCTCTGGTTTCACTTTCATTGACGCCTGGATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG14270.12506595103584756No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14230.12471538074562795No Hit
AAGTATGACAGTGCGCCCGCCACTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAACTGTGGCTGCACCAT14060.12322545701219459No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13980.12252431643175535No Hit
CTGTCATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC13630.11945682639233372No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13240.11603876606269248No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13060.11446119975670423No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12930.11332184631349046No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC12780.11200720772516692No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12690.11121842457217279No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12580.11025435627406883No Hit
GATCTATTCTGCATCGACTTTGCAATTAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA12400.10867678996808057No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12040.10552165735610404No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11870.10403173362267068No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11740.10289238017945693No Hit
ATTCTGCATCGACTTTGCAATTAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA11710.10262945246179221No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATGACAGTGCG11700.10254180988923732No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11680.10236652474412751No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11670.1022788821715726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA259.422278E-486.91164470
GTATCAA30750.038.9495161
GTGGTAT4000.038.0624281
ATCAACG36350.032.9548233
AACGCAG37350.032.255116
TCAACGC37250.032.1600074
CAACGCA37550.031.9917895
TATCAAC37750.031.6402422
ACGCAGA38850.031.009747
CGCAGAG38950.030.7563618
AGAGTAC38750.030.29847311
CACCGTT500.00516814629.9748767
GCAGAGT39750.029.9670989
CAGAGTA39550.029.94619810
GTGGCCG11200.029.09988270
AATGGTA2252.8398063E-828.9705570
CGGGTAT606.0576573E-428.21046817
ACCGTTT600.00954809526.43316368
CGAAACC1059.93723E-525.7277269
GGAAACG1450.001232971724.97461170
Produced by FastQC (version 0.11.5)