FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600881
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCGGTGTCCTCGTCTCTCAGACTGTCCATTTGCAGATAAA22220.36979035782459424No Hit
CTTTTTACCCTCACCATCAGCAGCCTGCAGCCTGAAGACATTGCAACTTATTATTGTCAACAATATCATGATCCA20230.3366723194775671No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19870.33068111656051696No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18860.3138724639321263No Hit
GGGTAAAAAGTGTCCCAGATTGACTTCCACTGAACCGTGAAGGGACCCCT18430.30671630489231644No Hit
GTTCAGTGGAAGTCAATCTGGGACACTTTTTACCCTCACCATCAGCAGCC16720.2782580910363283No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG15930.2651107290794683No Hit
GTGTATTACTGTGCGAGAGATCTGTGGGGTGACCACGCTTCGTACTGGGGCCAGGGAACGCTGGTCACCGTCTC15250.25379401245837363No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14790.24613858650880957No Hit
GTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGGCATTAGCAACTATTTGAATTGGTATCAGCAGAAA13500.22467010938937992No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13190.21951101798858677No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12110.20153740923743638No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11620.19338271637811813No Hit
CCACTGAACCGTGAAGGGACCCCTGTCTCCAAAATGGCGGCATCGTAGAT11340.18872289188707914No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11300.18805720267407358No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATC11250.1872250911578166No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11240.1870586688545652No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10830.18023535442125813No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10780.17940324290500115No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10780.17940324290500115No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10540.17540910762696774No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10260.17074928313592874No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9900.1647580802188786No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9600.15976541112133683No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGGGTAAAAAGTGTCCCAGATTGACTTCCACTGAACCGTGAAGGGAC9290.15460631972054367No Hit
GTTTAGGGGGTTTCCCTGGTTTCTGCTGATACCAATTCAAATAGTTGCTA8550.14229106927994062No Hit
CAGTAATACACAGCGGTGTCCTCGTCTCTCAGACTGTCCATTTGCAGATA8420.14012757933767253No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTAGGTTCTGGATGCACTGGGTCCGCCAAGTTCCAGGGAAGGGG8410.13996115703442114No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8310.1382969340019072No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT8240.13713197787914744No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8120.13513491024013075No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7840.13047508574909175No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC7730.12864444041332643No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG7520.12514957204504717No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7450.12398461592228745No Hit
CCTTCGGCCAAGGGACCCGCCTGGAGATTAGACGAACTGTGGCTGCACCA7360.1224868151930249No Hit
GTCTCCGTCCTCCCTGTCTGCCTCTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGGCATTAGCAA7340.12215397058652214No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7210.11999048064425402No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7170.11932479143124845No Hit
GCCTGAAGACATTGCAACTTATTATTGTCAACAATATCATGATCCACCTCCGATCACCTTCGGCCAAGGGACCCG7160.11915836912799706No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCTGATGACCCAGTCTCCGTCCTCC7100.11815983530848871No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7040.11716130148898035No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6860.11416570003045527No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6830.1136664331207011No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT6810.11333358851419832No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT6810.11333358851419832No Hit
CCCCCTAAACTCCTGATCTACGATGCCGCCATTTTGGAGACAGGGGTCCCTTCACGGTTCAGTGGAAGTCAAT6780.11283432160444415No Hit
GTATTACTGTGCGAGAGATCTGTGGGGTGACCACGCTTCGTACTGGGGCC6770.11266789930119275No Hit
ATCCACCTCCGATCACCTTCGGCCAAGGGACCCGCCTGGAGATTAGACGA6590.10967229784266767No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6580.10950587553941629No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTAATCTCCAGGCGGGTCC6550.1090066086296621No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6520.10850734171990793No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6490.10800807481015375No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6440.1071759632938968No Hit
CTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGGCATTAGC6250.10401393953212033No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6220.10351467262236615No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6150.1023497164996064No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6120.10185044958985223No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6100.10151760498334945No Hit
GAGGTGGATCATGATATTGTTGACAATAATAAGTTGCAATGTCTTCAGGC6080.10118476037684666No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6050.10068549346709248No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6040.1005190711638411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT200.00762691151.28118
TCTGGAG7450.048.34136670
GTGGTAT1650.043.518481
TCGTATG2100.042.73623740
ACATGCG2200.042.01320327
CGGTACC758.240022E-1041.6346645
ATCTCGT2000.041.2859937
GCGCAGA2250.041.16189631
ATGCGCA2250.041.12069329
CATGCGC2250.041.10355428
CGTATGC2300.040.57535641
CGCGGTA801.5752448E-938.96667543
ATGCCGT2250.038.52458644
CGAGACA2400.038.48003823
ACGAGAC2400.038.4704322
GCCGTCT2350.038.44175346
GATCTCG2150.038.37664836
TAACCGT753.508103E-837.3683553
CGCAGAT2400.037.1879932
CCCACGA2300.037.169519
Produced by FastQC (version 0.11.5)