FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600881
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23200.38609974354323073No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20770.3456591238531423No Hit
GTAATACACAGCGGTGTCCTCGTCTCTCAGACTGTCCATTTGCAGATAAA19890.33101396116701975No Hit
CTTTTTACCCTCACCATCAGCAGCCTGCAGCCTGAAGACATTGCAACTTATTATTGTCAACAATATCATGATCCA18620.30987832865409287No Hit
GGGTAAAAAGTGTCCCAGATTGACTTCCACTGAACCGTGAAGGGACCCCT17810.29639812209073013No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG17240.2869120508054007No Hit
GTGTATTACTGTGCGAGAGATCTGTGGGGTGACCACGCTTCGTACTGGGGCCAGGGAACGCTGGTCACCGTCTC16280.270935509693267No Hit
GTTCAGTGGAAGTCAATCTGGGACACTTTTTACCCTCACCATCAGCAGCC15280.25429327936812784No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14060.23398975837145788No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13880.23099415691293285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13830.23016204539667587No Hit
GTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGGCATTAGCAACTATTTGAATTGGTATCAGCAGAAA13440.22367157556987155No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11580.19271702716511255No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11500.19138564873910144No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11340.18872289188707914No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11110.18489517891229712No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10730.1785711313887442No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10050.1672544147676495No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATC9630.160264678031091No Hit
CCACTGAACCGTGAAGGGACCCCTGTCTCCAAAATGGCGGCATCGTAGAT9530.15860045499857708No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGGGTAAAAAGTGTCCCAGATTGACTTCCACTGAACCGTGAAGGGAC9280.15443989741729228No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9280.15443989741729228No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8960.14911438371324773No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTAGGTTCTGGATGCACTGGGTCCGCCAAGTTCCAGGGAAGGGG8880.1477830052872366No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8740.14545309304171708No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8720.1451202484352143No Hit
GTTTAGGGGGTTTCCCTGGTTTCTGCTGATACCAATTCAAATAGTTGCTA8640.14378887000920315No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8300.1381305116986558No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8160.1358005994531363No Hit
GTCTCCGTCCTCCCTGTCTGCCTCTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGGCATTAGCAA8110.13496848793687935No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT7620.1268137950775611No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7510.12498314974179581No Hit
GCCTGAAGACATTGCAACTTATTATTGTCAACAATATCATGATCCACCTCCGATCACCTTCGGCCAAGGGACCCG7370.12265323749627631No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7280.12115543676701378No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7210.11999048064425402No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7030.11699487918572894No Hit
CCTTCGGCCAAGGGACCCGCCTGGAGATTAGACGAACTGTGGCTGCACCA6970.11599634536622061No Hit
CAGTAATACACAGCGGTGTCCTCGTCTCTCAGACTGTCCATTTGCAGATA6890.11466496694020946No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6840.1138328554239525No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6770.11266789930119275No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCTGATGACCCAGTCTCCGTCCTCC6730.11200221008818717No Hit
GTATTACTGTGCGAGAGATCTGTGGGGTGACCACGCTTCGTACTGGGGCC6720.11183578778493578No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6670.11100367626867882No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG6630.11033798705567324No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT6610.11000514244917046No Hit
CCCCCTAAACTCCTGATCTACGATGCCGCCATTTTGGAGACAGGGGTCCCTTCACGGTTCAGTGGAAGTCAAT6580.10950587553941629No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTAATCTCCAGGCGGGTCC6570.10933945323616491No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6560.1091730309329135No Hit
GAGGTGGATCATGATATTGTTGACAATAATAAGTTGCAATGTCTTCAGGC6540.10884018632641072No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT6490.10800807481015375No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC6410.10667669638414262No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6380.10617742947438844No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6260.10418036183537172No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6210.10334825031911477No Hit
GTGCAGCCACAGTTCGTCTAATCTCCAGGCGGGTCCCTTGGCCGAAGGTG6190.10301540571261197No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6150.1023497164996064No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6030.10035264886058971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)