FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926091
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTTACTCTCCCCGGTACACTTTTGGCCAGGGGACCAAGCTGG168510.8748807818529861No Hit
GTATAATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC150850.7831924867516644No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCTTGATCTATAAGGTGT91530.4752111919945631No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT84530.43886815316617955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC75090.38985696937475955No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57660.29936280269208465No Hit
CCTTATAGATCAAGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA52000.2699768598679917No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC51990.269924941241094No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49010.2544531904255822No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC47710.2477037689288824No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG47650.24739225716749624No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG47300.24557510522607706No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG45070.23399725142789202No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG44850.23285504163614285No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAA43850.22766317894637378No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43670.22672864366221532No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42900.22273090939109313No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA42890.22267899076419545No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA42660.22148486234554857No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATTATACTTA42660.22148486234554857No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA42520.2207580015689809No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG41740.21670834867096103No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41460.2152546271178257No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT39960.2074668330831721No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCT37310.19370839695528405No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACAT37120.19272194304422793No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36290.1884126970117196No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA35910.18643978918960735No Hit
TCTATAAGGTGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT35820.18597252154752814No Hit
GGCCAAAAGTGTACCGGGGAGAGTAAGTATAATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG34950.18145560100742902No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT34390.17854815790115836No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCC33340.17309670207690084No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32540.16894321192508557No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31680.16447821001188417No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30620.15897483556072894No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30310.15736535812690056No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT29500.1531599493481876No Hit
GTAATATATGGCGGTGTCCGGGGCCTTCAGGCTGCTCCACTGTAGGTAGG29210.15165430916815456No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC29200.15160239054125688No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29180.1514985532874615No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27250.1414782582962072No Hit
TACTTACTCTCCCCGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT27030.140336048504458No Hit
GAGTAAGTATAATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG26810.1391938387127088No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG26680.1385188965630388No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26610.138155466174755No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT26410.13711709363680116No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26370.1369094191292104No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC25740.13363854563465588No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25210.1308868584090783No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24250.1259026702269No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT24110.1251758094503323No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24080.12502005356963924No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23080.11982819087987016No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22850.11863406246122327No Hit
ATTATACTTACTCTCCCCGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT22780.11827063207293943No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22710.1179072016846556No Hit
ACTTATTACTGCCAACACTATTATACTTACTCTCCCCGGTACACTTTTGG22690.11780336443086023No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22380.11619388699703181No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG22070.1145844095632034No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22060.11453249093630571No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACACCTTATAGATCAAGAGCTTAGGGGCTTTCCCTGGTTTTTG21990.11416906054802188No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21860.1134941183983519No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTG21700.11266342036798883No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21650.11240382723350038No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21540.1118327223376258No Hit
CCCCTAAGCTCTTGATCTATAAGGTGTCTAGTTTAGAAAGTGGGGTCCCA21520.1117288850838304No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21480.11152121057623965No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21140.10975597726171818No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21110.1096002213810251No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA21100.10954830275412739No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21060.10934062824653663No Hit
GCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGCTACTA20560.1067446969016521No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20550.10669277827475442No Hit
GTACCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA20170.10471987045264217No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19870.10316231164571145No Hit
ATATATTACTGTGCGAGACCCGCGACGATGACTCCCGATCCTTTTGATAT19830.10295463713812067No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19610.10181242734637148No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19560.10155283421188303No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19540.10144899695808765No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC19480.10113748519670152No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19280.1000991126587477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG350.003827876961.04273270
CGTCGTA252.4164785E-455.88199248
TATCGCG1750.046.89621436
GCGTGTA551.1465818E-745.32084756
GTATCGC1850.044.3077535
AGTATCG3050.042.49108534
CGTATGC6500.041.37978741
CTCTTAT3300.040.9932481
ACCGATC12150.040.98760627
CTTATAC12900.040.6357541
GACTGAG12150.040.4439570
CGCGTAT1200.040.08278739
CGATCAG12550.040.01363829
TATCTCG7500.039.66637836
ACGAGAC12900.039.32696522
TCGTATG6400.039.25437540
GACCGAT12800.039.1555926
ATCTCGT7000.039.12950537
TATACAC13450.038.951813
TGCACCA68100.038.79784470
Produced by FastQC (version 0.11.5)