FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926091
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC161410.8380185567556258No Hit
CTATTATACTTACTCTCCCCGGTACACTTTTGGCCAGGGGACCAAGCTGG161280.8373436146059559No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105340.546910815740274No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT85480.4438004227214602No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCTTGATCTATAAGGTGT81550.4233964023506677No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58750.30502193302393293No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55890.29017320573119343No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54550.28321610972690286No Hit
CCTTATAGATCAAGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA52250.271274825540434No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC48750.2531033061262422No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47150.2447963258226117No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC41630.21613724377508642No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41200.2139047428184857No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATTATACTTA40880.21224334675775963No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40390.20969933403977278No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40200.20871288012871667No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA39790.20658421642591132No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39540.2052862507534691No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA38740.2011327606016538No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG38140.19801764298779237No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38030.19744653809191776No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAA37910.1968235145691455No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37800.1962524096732709No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG35940.1865955450703004No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35290.18322083432195052No Hit
GGCCAAAAGTGTACCGGGGAGAGTAAGTATAATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG34220.17766554124389763No Hit
TCTATAAGGTGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT33490.1738754814803662No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33120.17195449228515164No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA32740.1699815844630394No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA32600.16925472368647174No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACAT32140.16686646684917794No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT31890.1655685011767357No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31880.16551658254983798No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31680.16447821001188417No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCC31560.16385518648911188No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31420.1631283257125442No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCT30990.16089582475594352No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29420.15274460033300608No Hit
GTAATATATGGCGGTGTCCGGGGCCTTCAGGCTGCTCCACTGTAGGTAGG27920.14495680629835248No Hit
GAGTAAGTATAATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG27870.144697213163864No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27800.14433378277558018No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26560.13789587304026651No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25780.13384622014224665No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25340.13156180055874828No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC25190.1307830211552829No Hit
TACTTACTCTCCCCGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT25090.130263834886306No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24940.12948505548284062No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24850.12901778784076143No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24430.1268372055110584No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24420.12678528688416071No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24390.12662953100346766No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24160.12543540258482075No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG23890.12403359965858311No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23290.12091848204472168No Hit
ATTATACTTACTCTCCCCGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT22940.1191013301033025No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGTACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG22650.11759568992326945No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22220.11536318896666875No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22200.11525935171287338No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22100.11474016544389648No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21940.11390946741353342No Hit
ACTTATTACTGCCAACACTATTATACTTACTCTCCCCGGTACACTTTTGG21520.1117288850838304No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21440.11131353606864888No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21220.1101713262768997No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTG21160.10985981451551355No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACACCTTATAGATCAAGAGCTTAGGGGCTTTCCCTGGTTTTTG21090.1094963841272297No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG20660.10726388317062902No Hit
ATATATTACTGTGCGAGACCCGCGACGATGACTCCCGATCCTTTTGATAT20640.10716004591683362No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20550.10669277827475442No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20470.1062774292595729No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20450.1061735920057775No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20270.10523905672161907No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG20150.1046160331988468No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20120.10446027731815372No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19970.10368149791468834No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19890.10326614889950682No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19780.10269504400363223No Hit
GCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGCTACTA19580.1016566714656784No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19580.1016566714656784No Hit
CCCCTAAGCTCTTGATCTATAAGGTGTCTAGTTTAGAAAGTGGGGTCCCA19540.10144899695808765No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG19310.10025486853944077No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGTACCTGGGAGCCAGAG19310.10025486853944077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5150.042.556771
TGCACCA68800.039.9228870
TACGTAT800.001765616637.31921470
GTATCAA41900.037.1783831
ACGGTAC558.596682E-636.82063319
ACCGGTA558.608953E-636.8129818
GACTGAG10550.035.3736670
TACTCGT400.002801016433.97130631
TATCAAC48050.032.2110142
ATCAACG48150.032.1432843
TGTGCGC450.003718197632.06282460
TCAACGC48300.032.0451244
AACGCAG49450.031.707396
CAACGCA49200.031.5951845
AATGGTA5950.031.36068370
GTCACCG11000.029.85537170
CGCAGAG52650.029.8442978
ACGCAGA52600.029.8085637
GACAGGT3300.029.6310211
CGACTGA9250.029.41435469
Produced by FastQC (version 0.11.5)