FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918047
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA66040.3443085596963995No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61910.3227762406239263No Hit
GTATAGTGACTGGCCTCTGGCGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT58170.30327723981737675No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46670.24332041915552644No Hit
GAGTATTAGCAACCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC41890.21839923630651387No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT41040.21396764521411624No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT40600.21167364511922804No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA40130.20922323592696113No Hit
CTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCT39670.2068249631004871No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37990.19806605364727767No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37650.19629341721031862No Hit
GTCACTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACT36630.19097550789944148No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGTCTCCCAGCCAGGTTCAGTGGCGGTGGGTCTGGGACAGAGTTCA34780.1813302802277525No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG34270.1786713255723139No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCAGTGGCCCTGCT34120.1778892800854202No Hit
GCAGTATAGTGACTGGCCTCTGGCGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT33590.17512605269839582No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCCAGAACTCATTGTATCTG33120.1726756435061289No Hit
GTGTATTACTGTGCGGGAGATTACGTTTGGCCACCTAAATACTACTACTA32900.1715286434586848No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31940.16652355234256513No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA31840.16600218868463598No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31440.16391673405291945No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAGTGACTGG31350.16344750676078323No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30530.15917232476576434No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30100.15693046103666908No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29520.15390655182068008No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC29190.15218605174951394No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAACCACTTAGCCTGGTA28770.1499963243862116No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA28500.14858864250980294No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAACCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT28030.146138233317536No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG27220.14191518768831005No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26930.14040323308031555No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTATAGCATGAATTGGG26570.13852632391177067No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG26020.13565882379316044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25950.13529386923261005No Hit
GTATTACTGTGCGGGAGATTACGTTTGGCCACCTAAATACTACTACTACGGTATGGACGTCTGGGGCCAAGGGAC25940.13524173286681712No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25480.13284346004034311No Hit
GTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAA25340.13211355091924235No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25120.13096655087179826No Hit
GTATATGTAAGTACTCTTACTACTAATGGATGAGACCCACTCAAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT25090.1308101417744195No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24960.13013236901911163No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT24840.12950673262959667No Hit
CCTAAATACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGT24370.12705632343732975No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC24280.12658709614519353No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT24040.1253358233661636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23730.12371959602658329No Hit
GATCAGCACTGAGCACCGGGGACTCACCATGGAACTGGGGCTCCGCTGGG23010.11996577768949354No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22940.11960082312894313No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22760.11866236854467069No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22610.117880323057777No Hit
GCACTGAGCACCGGGGACTCACCATGGAACTGGGGCTCCGCTGGGTTTTC22370.11662905027874709No Hit
GTGTATTACTGTGCGGGAGATTACGTTTGGCCACCTAAATACTACTACTACGGTATGGACGTCTGGGGCCAAGGG22340.11647264118136835No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGCC22270.11610768662081794No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22190.11569059569447465No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG22170.11558632296288882No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22070.11506495930495968No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCC21680.11303164103903607No Hit
GTTTATTACTGTCAGCAGTATAGTGACTGGCCTCTGGCGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAA21480.1119889137231778No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21440.11178036826000615No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGG21400.11157182279683449No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGT21240.11073764094414787No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21190.1104769591151833No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCA20870.10880859540981008No Hit
GAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTC20650.10766159536236598No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20650.10766159536236598No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20260.10562827709644237No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19890.10369923156210459No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19580.10208300422252428No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19460.1014573678330093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)