FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918047
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94670.4935749749615103No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61030.31818824043414995No Hit
GTATAGTGACTGGCCTCTGGCGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT55960.29175510297714286No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA54700.2851859208872358No Hit
GAGTATTAGCAACCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC42090.21944196362237212No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT40670.21203859967977842No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39930.20818050861110285No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38650.20150705378960995No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36450.19003705331516904No Hit
CTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCT36170.18857723507296745No Hit
GTCACTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACT35690.1860746895149076No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34370.17919268923024306No Hit
GTGTATTACTGTGCGGGAGATTACGTTTGGCCACCTAAATACTACTACTA33300.17361409809040135No Hit
GCAGTATAGTGACTGGCCTCTGGCGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT33210.1731448707982651No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32450.16918250699800372No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32010.16688850690311552No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCAGTGGCCCTGCT31360.16349964312657614No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAGTGACTGG31170.1625090521765108No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGTCTCCCAGCCAGGTTCAGTGGCGGTGGGTCTGGGACAGAGTTCA29880.15578346098922496No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCCAGAACTCATTGTATCTG29580.15421937001543756No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAACCACTTAGCCTGGTA29470.15364586999171553No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29090.15166468809158482No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAACCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT29030.15135186989682733No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG28420.14817155158345963No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC27930.14561686965960688No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27770.14478268780692027No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27530.14353141502789035No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26830.13988186942238642No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTATAGCATGAATTGGG26230.13675368747481162No Hit
GTATTACTGTGCGGGAGATTACGTTTGGCCACCTAAATACTACTACTACGGTATGGACGTCTGGGGCCAAGGGAC25540.1331562782351006No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA25280.13180073272448486No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24910.12987168719014705No Hit
GTGTATTACTGTGCGGGAGATTACGTTTGGCCACCTAAATACTACTACTACGGTATGGACGTCTGGGGCCAAGGG24650.1285161416795313No Hit
GTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAA24530.12789050529001636No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24060.12544009609774942No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23520.12262473234493211No Hit
GTATATGTAAGTACTCTTACTACTAATGGATGAGACCCACTCAAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT23170.12079995954218015No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23040.12012218678687228No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23000.11991364132370062No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22620.1179324594235699No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGT22200.11574273206026757No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22050.11496068657337384No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC22000.11470000474440929No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGCC22000.11470000474440929No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21850.11391795925751559No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21760.11344873196537937No Hit
GATCAGCACTGAGCACCGGGGACTCACCATGGAACTGGGGCTCCGCTGGG21750.11339659559958645No Hit
GTTTATTACTGTCAGCAGTATAGTGACTGGCCTCTGGCGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAA21710.1131880501364148No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21180.11042482274939039No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20980.10938209543353214No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT20880.10886073177560299No Hit
GAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTC20860.10875645904401716No Hit
GCACTGAGCACCGGGGACTCACCATGGAACTGGGGCTCCGCTGGGTTTTC20680.10781800445974472No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCA20410.10641032258333608No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20290.1057846861938211No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGG20260.10562827709644237No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20000.10427273158582662No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19990.10422059522003371No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19870.10359495883051875No Hit
CCTAAATACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGT19860.10354282246472583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG200.007881504550.861184
GGTATCA14250.044.4844931
TCTGGAG22350.040.66405570
GTATCAA31900.035.598861
AATGGTA4850.030.9735670
TATCAAC38450.029.4525342
ATCAACG39350.028.8673273
TCAACGC39700.028.5266724
TGTTAGA13800.028.3026570
AACGCAG40400.028.200266
CAACGCA40200.028.0875195
CGCAGAG41950.027.319958
GCAGAGT44600.025.696689
ATACAAT22250.025.44537446
GCAGATA22050.025.3870542
CGTTGTC22250.025.25412863
GTTGTCT22650.025.20440964
GATACAA22650.025.11446245
CAGTTCG24950.024.98678670
ATTTGCA22400.024.83327338
Produced by FastQC (version 0.11.5)