FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359177
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG74830.5505537542203848No Hit
GATTATTACTGTGCAACATGGGATGACAGTCTGAATGGTCGGGTGTTCGG71340.5248764509699619No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC40300.29650295730430987No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCGCAGCGTCTGGGACCC39220.2885569723442936No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCG38300.2817881703413168No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35950.26449829565980004No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30310.22300259642415965No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCGCAGCGTCT29170.21461516785525359No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC29000.21336441096339917No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCGACCATTCAGA28920.21277581948487947No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28710.21123076685376518No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG28070.20652203502560743No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27960.20571272174264277No Hit
GTAATATACGGCCGTGTCCTCGGTTCTCAGGCTGTTCATTTGCAGATACA27350.20122471171892992No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23090.16988221548775473No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22900.16848431072627038No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22540.16583564907293163No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21950.1614947869188487No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC21870.16090619544032897No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21480.15803681198254532No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21330.15693320296032084No Hit
GTGCTGACTCAGCCACCCGCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21110.1553145763943916No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20840.15332808015438754No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20790.15296021048031272No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20560.15126800997956852No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20260.14906079193511956No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20200.14861934832622975No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19680.1447935037158516No Hit
CCCCTGAGGGCCGCTGATCATTTTTATAGATGAGGAGTTTGGGGGCCGTACCTGGGAATTGCTGGTACCAGTTTA18970.13956975434398905No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18920.13920188466991423No Hit
GTATATTACTGTGCTAGAGCCAGCCTGAATTACTATGATGGTGGTGTTTTTCGCTCCTGGGGGGGGATTGACTA18900.1390547368002843No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG18760.13802470171287476No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT18750.13795112777805982No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18460.1358174836684258No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17710.13029943855730342No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17510.12882795986100412No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACAGTCTGAATGGTCGGGTGTTCGGCGGAGGGAC17120.12595857640322047No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16830.12382493229358647No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16600.12213273179284229No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC16380.12051410522691305No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCATAAGTCATACTGTAAA16340.1202198094876532No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA16290.11985193981357836No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT16270.11970479194394842No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15780.11609966913801513No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15400.11330385961504645No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC15360.1130095638757866No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCGACCATTCAGAC15260.11227382452763694No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15200.11183238091874714No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14970.11014018041800296No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14960.11006660648318799No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14770.10866870172170365No Hit
CTGTAAACTGGTACCAGCAATTCCCAGGTACGGCCCCCAAACTCCTCATC14680.10800653630836897No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14530.10690292728614448No Hit
CTCCTGTGCAGCCTCCGGATTCACCTTCAGTGGCCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG14520.10682935335132952No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCATAAGTCATACTGTAAACTGGTACCAGCAATTCCCAGGTACGGCCCC14070.10351852628465608No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14030.10322423054539623No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13830.10175275184909692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)