FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359177
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGTCTGAATGGTCGGGTGTTCGG70540.5189905361847648No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCGCAGCGTCTGGGACCC41980.308863378353224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39850.29319213023763646No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39230.2886305462791086No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38680.2845839798642855No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG36250.26670551370424894No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCG34920.25692018037385855No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCGCAGCGTCT30480.22425335331601404No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27550.20269619041522918No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27130.19960608515300066No Hit
GTAATATACGGCCGTGTCCTCGGTTCTCAGGCTGTTCATTTGCAGATACA26720.1965895538255871No Hit
GTGCTGACTCAGCCACCCGCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC25180.18525916786408245No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25080.1845234285159328No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC24910.1832726716240784No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24680.1815804711233342No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG24150.17768105257814104No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23520.17304589468479822No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCGACCATTCAGA22850.16811644105219556No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22320.1642170225070024No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22180.1631869874195929No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC21630.1591404210047698No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20940.1540638195025372No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20730.15251876687142293No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20370.14987010521808417No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19850.14604426060770598No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19790.1456028169988162No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19560.143910616498072No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19360.14243913780177267No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCATAAGTCATACTGTAAA18970.13956975434398905No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18910.13912831073509926No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC18900.1390547368002843No Hit
CCCCTGAGGGCCGCTGATCATTTTTATAGATGAGGAGTTTGGGGGCCGTACCTGGGAATTGCTGGTACCAGTTTA18500.13611177940768568No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACAGTCTGAATGGTCGGGTGTTCGGCGGAGGGAC18310.13471387464620135No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18190.13383098742842175No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18140.13346311775434694No Hit
GTATATTACTGTGCTAGAGCCAGCCTGAATTACTATGATGGTGGTGTTTTTCGCTCCTGGGGGGGGATTGACTA18110.13324239594990203No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG18020.13258023053656737No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCATAAGTCATACTGTAAACTGGTACCAGCAATTCCCAGGTACGGCCCC17870.13147662151434286No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT17340.1275772029691497No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT17290.1272093332950749No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17060.1255171327943307No Hit
CTGTAAACTGGTACCAGCAATTCCCAGGTACGGCCCCCAAACTCCTCATC16590.12205915785802732No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15740.11580537339875528No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15410.11337743354986143No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14890.10955158893948323No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14660.10785938843873902No Hit
CTCCTGTGCAGCCTCCGGATTCACCTTCAGTGGCCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG14560.10712364909058938No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14560.10712364909058938No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14560.10712364909058938No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14110.10381282202391595No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13950.1026356390668765No Hit
GTCCATGTAGTGGCCACTGAAGGTGAATCCGGAGGCTGCACAGGAGAGTCTCAGGGACCCTCCAGACTGGACCAA13910.10234134332761664No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13740.10109058643576223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)