FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374832
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT46280.33662294738557147No Hit
CTATTAAGCTTCATCAAGTAACGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC45320.3296402760482735No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACTAG44900.32658535733820565No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44820.3260034680600975No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39990.29087190289431725No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33180.24133857809536No Hit
GACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACTAGAATGCCAGTGCCCCAAGTCTGACAGTAATAGTCAG32090.2334103366811363No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31510.22919163941485213No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTGGGTCCCTC31120.22635492918407488No Hit
GACTATTACTGTCAGACTTGGGGCACTGGCATTCTAGTATTCGGCGGAGGGACCCAGCTGACCGTCCTAGGTCAG28020.2038067196573836No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27240.19813329919582903No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCCGGAGATGGTGAGGTAGC26880.1955147974443423No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25830.18787750066917266No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCAC25780.18751381987035506No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24570.17871274453896913No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT24530.17842179989991505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23810.1731847963969416No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23580.17151186472238064No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23320.1696207245685291No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23100.16802052905373166No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22880.1664203335389342No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22310.16227437243241358No Hit
GCCCCAGAACTAGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT21830.1587830367637646No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21750.15820114748565645No Hit
GTTCTGGGGCTGAGCGCTACCTCACCATCTCCGGCCTCCAGTCTGAGGAT21470.15616453501227787No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21450.15601906269275084No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21230.1544188671779534No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21010.15281867166315594No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAA20980.1526004631838654No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20820.15143668462764906No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20070.14598147264538505No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCTAGTTCTGGGGCTGAGCGCTACCTCACCATCTCCGG19630.14278108161579015No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19530.14205372001815494No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGGAATTACTACTGGAGCTGGAACCG19510.14190824769862792No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19490.14176277537910087No Hit
GAACTAGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCTGTGGCT19420.14125362226075622No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19270.14016257986430342No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19000.1381987035506884No Hit
CTATTACTGTCAGACTTGGGGCACTGGCATTCTAGTATTCGGCGGAGGGACCCAGCTGACCGTCCTAGGTCAGCC18860.1371803973139991No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACTTGGGGCACTGGCATTCTAGTATTCGGCGGAGGGACCCAG18560.13499831252109348No Hit
TCCTCAGACTGGAGGCCGGAGATGGTGAGGTAGCGCTCAGCCCCAGAACTAGAGCCTGAGAAGCGATCAGGGATC18400.13383453396487716No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18340.13339811700629603No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17730.12896121126072133No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC17470.1270700711068698No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC17380.1264154456689981No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCCGGAGATGGTGAGGTAGCGCTCAGCCCCAGAACTAGAGC17360.12626997334947107No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16870.12270590152105858No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16620.12088749752697056No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA16340.118850885053592No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT16210.11790531497666625No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCACCTGCACT16050.11674153642044992No Hit
CTCTAGTTCTGGGGCTGAGCGCTACCTCACCATCTCCGGCCTCCAGTCTG16010.11645059178139584No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG15870.11543228554470655No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15740.11448671546778078No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15650.11383209002990911No Hit
ATAATATACGGCCGTGTCCGCGGCAGTCACGGAGTTCAGTTTCAGGGAGA15630.11368661771038206No Hit
ACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACTAGAATGCCAGTGCCCCAAGTCTGACAGTAATAGTCAGC15330.11150453291747646No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15200.1105589628405507No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT15130.11004980972220606No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT14450.10510375085828669No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC14140.10284892990561754No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTGGGTC14040.10212156830798236No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13940.10139420671034716No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13770.10015769199436732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG259.399368E-486.9670370
AGGGGGG3850.062.11931270
TGATCGA450.00965203848.31501870
CGTCTTT450.00965203848.31501870
GGTATCA9650.047.6036341
TCGGCTT404.4513472E-543.98637453
TCGTATG3150.040.46383740
AATCGCG955.456968E-1239.70833636
CGAGACG5450.039.3291423
TTGGGCG350.001445479138.8719184
TATACAC5850.038.373563
ACGAGAC5500.038.35161222
CACGAGA5500.038.3446421
CGCGTAT451.01945414E-438.21888439
GTATCAA25350.037.182231
CTTATAC5950.037.173211
ATCGCGT655.678612E-736.9613537
CGTATGC3650.036.8559841
ATGAATC5250.036.47147433
CGCTCAT5800.036.43816428
Produced by FastQC (version 0.11.5)