FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374832
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57050.4149597914508827No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45490.33087679076425336No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCAC43650.31749333736776564No Hit
GCTTAATAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT41510.3019277991783723No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACTAG39650.2883988734623576No Hit
CTATTAAGCTTCATCAAGTAACGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC37250.2709421951191127No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36940.2686873741664436No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35950.2614864943498551No Hit
GACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACTAGAATGCCAGTGCCCCAAGTCTGACAGTAATAGTCAG29690.21595365833789149No Hit
GACTATTACTGTCAGACTTGGGGCACTGGCATTCTAGTATTCGGCGGAGGGACCCAGCTGACCGTCCTAGGTCAG27710.20155189870471446No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTGGGTCCCTC27660.20118821790589686No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27260.19827877151535606No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26570.19325997649167317No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26150.19020505778160532No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24780.18024020389400305No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT24490.17813085526086095No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAA24110.1753668811898472No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24080.17514867271055662No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23550.17129365624309006No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23510.171002711604036No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCACCTGCACT23350.16983893304781966No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22770.16562023578153548No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCCGGAGATGGTGAGGTAGC22750.16547476346200846No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22390.16285626171052173No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21770.15834661980518347No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21410.15572811805369674No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC20770.15107300382883143No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20680.15041837839095976No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20420.14852723823710826No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACTTGGGGCACTGGCATTCTAGTATTCGGCGGAGGGACCCAG20220.14707251504183785No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20020.14561779184656742No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19440.14139909458028327No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT19340.14067173298264807No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGGAATTACTACTGGAGCTGGAACCG19320.14052626066312102No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19280.14023531602406694No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19000.1381987035506884No Hit
GTTCTGGGGCTGAGCGCTACCTCACCATCTCCGGCCTCCAGTCTGAGGAT18880.13732586963352614No Hit
CTATTACTGTCAGACTTGGGGCACTGGCATTCTAGTATTCGGCGGAGGGACCCAGCTGACCGTCCTAGGTCAGCC18600.13528925716014756No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18220.1325252830891338No Hit
GCCCCAGAACTAGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT18210.13245254692937028No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17970.1307068790950458No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA17600.12801564118379555No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCTAGTTCTGGGGCTGAGCGCTACCTCACCATCTCCGG17470.1270700711068698No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17320.125979028710417No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17040.12394241623703842No Hit
GAACTAGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCTGTGGCT16630.12096023368673409No Hit
TCCTCAGACTGGAGGCCGGAGATGGTGAGGTAGCGCTCAGCCCCAGAACTAGAGCCTGAGAAGCGATCAGGGATC16450.11965098281099071No Hit
ATAATATACGGCCGTGTCCGCGGCAGTCACGGAGTTCAGTTTCAGGGAGA16420.11943277433170017No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC16270.11834173193524737No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16140.1173961618583216No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16060.11681427258021344No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15870.11543228554470655No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15640.1137593538701456No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC15490.11266831147369279No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15410.11208642219558464No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG15140.11012254588196957No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT15140.11012254588196957No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15110.109904337402679No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC15060.10954065660386142No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14950.10874055884646269No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCCGGAGATGGTGAGGTAGCGCTCAGCCCCAGAACTAGAGC14780.10750404413048285No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14280.10386723614230683No Hit
ACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACTAGAATGCCAGTGCCCCAAGTCTGACAGTAATAGTCAGC14220.10343081918372574No Hit
CTCTAGTTCTGGGGCTGAGCGCTACCTCACCATCTCCGGCCTCCAGTCTG14200.10328534686419868No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG13970.10161241518963772No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13930.10132147055058364No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13930.10132147055058364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)