FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223052
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139061.1369917223470467No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32830.26842685347802053No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30920.252810183050271No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28860.23596707253657245No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26230.21446348969626802No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24050.19663922711381038No Hit
GATCTATGCTGCATCCATTTTACAAAATGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA22260.18200370875481992No Hit
GAGTTACGTTACCCCTCGAACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT22160.18118608203085396No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21840.17856967651416294No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21690.177343236428214No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19870.16246243005203376No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18440.15077036789932072No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17800.14553755686593867No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17650.14431111677998973No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17090.13973240712578042No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17060.13948711910859063No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16840.13768834031586555No Hit
GATTTCACTCTGACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACGTTACC16580.13556251083355408No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16420.13425430807520858No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16330.13351844402363922No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15930.13024793712777544No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15920.13016617445537884No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCA15330.12534217678397974No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14610.11945526437142492No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14460.118228824285476No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14170.11585770678597476No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13670.11176957316614501No Hit
ACTTACTACTGTCAACAGAGTTACGTTACCCCTCGAACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA13270.10849906627028122No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13150.10751791420152208No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12950.1058826607535902No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12830.10490150868483106No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12580.10285744187491619No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12550.1026121538577264No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12540.10253039118532982No Hit
ACGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTCAGAGTGAAATCTGTC12480.10203981515095024No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC12470.10195805247855366No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12300.10056808704781155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG553.807145E-948.06282
CTAACGC651.6636477E-840.675183
GTGGTAT5950.039.4296721
GTATCAA33900.035.2850461
TAAGGCG450.00570502529.3753175
ATCAACG40900.029.0892163
AACGCAG41550.029.030666
TCAACGC41100.029.0280744
CAACGCA41650.028.9609575
TATCAAC41300.028.8027672
AGAGTAC43450.028.1357311
CGCAGAG43200.027.9983488
ATCGGTC550.00724540527.97198965
ACGCAGA43750.027.6463687
GCAGAGT44100.027.5768269
CAGAGTA49950.024.54559110
GATTCAC2500.023.7910861
AACGCCA1152.4679357E-622.9893785
TGTTAGA19100.022.95979970
GAGTACG42200.022.70460912
Produced by FastQC (version 0.11.5)